Interaction Details


Details of experiments between Q5EBL8 and Q8N743
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: PDZD11
Fragment: 37-128
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: KIR3DL3
Fragment: 401-410
Site: PBM
Sequence: PKTPPTDTSV
Construct: biotin-ttds-PKTPPTDTSV
Average holdup BI: 0.16
Immobilized partner concentration (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
Measured pKd: 4.3
PUBMED ID: 36115835