Interaction Details


Details of experiments between Q53GG5 and P03409
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: PDLIM3
Fragment: 1-94
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HTLV1-TAX1
Fragment: 344-353
Site: PBM
Sequence: SEKHFRETEV
Construct: biotin-ttds-SEKHFRETEV
Average holdup BI: 0.19
Holdup BI standard deviation: 0.22
Immobilized partner concentration (10-6M): 19
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 2
Measured pKd: 4.12
PUBMED ID: 36115835