Interaction Details


Details of experiments between Q53ET0 and Q04917
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: CRTC2
Fragment: 122-131
Site: 14-3-3_pSer127
Modification: (SEP)127
Sequence: TRHIDSSPYS
Construct: TRHID(pSer)SPYS (crude synthetic peptide)

Protein B

Protein: YWHAH
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3eta
Average holdup BI: 0.43
Holdup BI standard deviation: 0.06
Immobilized partner concentration (10-6M): 130
P value: 5.16
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 3.77
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 2

Protein A

Protein: CRTC2
Fragment: 159-168
Site: 14-3-3_pSer164
Modification: (SEP)164
Sequence: LFRLPSALNR
Construct: LFRLP(pSer)ALNR (crude synthetic peptide)

Protein B

Protein: YWHAH
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3eta
Average holdup BI: 0.13
Holdup BI standard deviation: 0.56
Immobilized partner concentration (10-6M): 130
P value: 0.19
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 3

Protein A

Protein: CRTC2
Fragment: 166-175
Site: 14-3-3_pSer171
Modification: (SEP)171
Sequence: LNRTSSDSAL
Construct: LNRTS(pSer)DSAL (crude synthetic peptide)

Protein B

Protein: YWHAH
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3eta
Average holdup BI: 0.49
Holdup BI standard deviation: 0.08
Immobilized partner concentration (10-6M): 130
P value: 4.27
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 3.86
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 4

Protein A

Protein: CRTC2
Fragment: 65-74
Site: 14-3-3_pSer70
Modification: (SEP)70
Sequence: SHYGGSLPNV
Construct: SHYGG(pSer)LPNV (crude synthetic peptide)

Protein B

Protein: YWHAH
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3eta
Average holdup BI: 0.81
Holdup BI standard deviation: 0.08
Immobilized partner concentration (10-6M): 130
P value: 6.34
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 4.52
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))