Interaction Details


Details of experiments between Q15758 and Q8TEW8
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: SLC1A5
Fragment: 532-541
Site: PBM
Sequence: TVASEKESVM
Construct: biotin-ttds-TVASEKESVM

Protein B

Protein: PARD3B
Fragment: 184-299
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.05
Holdup BI standard deviation: 0
Normalized holdup BI: 0.07
Normalized holdup BI standard deviation: 0
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 2
Measured pKd: 3.61
PUBMED ID: 36115835