Interaction Details


Details of experiments between Q15758 and O14907
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: SLC1A5
Fragment: 532-541
Site: PBM
Sequence: TVASEKESVM
Construct: biotin-ttds-TVASEKESVM

Protein B

Protein: TX1BP3
Fragment: 7-124
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.07
Holdup BI standard deviation: 0.04
Normalized holdup BI: 0.08
Normalized holdup BI standard deviation: 0.04
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 2
Measured pKd: 3.69
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: SLC1A5
Fragment: 1-541
Construct: endogenous protein (Jurkat)

Protein B

Protein: TX1BP3
Fragment: 7-124
Site: PDZ
Construct: avi(biotin)-his6-MBP-TEVsite-PDZ
Average holdup BI: -0.09
Immobilized partner concentration (10-6M): 10
P value: 1.16
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-08-03
Number of measurements: 3
The affinity was below the detection threshold of the assay.
PUBMED ID: 36542723
Experimental Details

-log10(P Two-sided unpaired T-test (6 vs 3))