Interaction Details


Details of experiments between Q15700 and Q6ZTQ3
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: DLG2
Fragment: 189-283
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: RASSF6
Fragment: 360-369
Site: PBM
Sequence: KLVIKTETTV
Construct: biotin-ttds-KLVIKTETTV
Average holdup BI: 0.76
Normalized holdup BI: 0.76
Immobilized partner concentration (10-6M): 20.7
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 5.24
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: DLG2
Fragment: 93-186
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: RASSF6
Fragment: 360-369
Site: PBM
Sequence: KLVIKTETTV
Construct: biotin-ttds-KLVIKTETTV
Average holdup BI: 0.4
Immobilized partner concentration (10-6M): 9
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
Measured pKd: 4.95
PUBMED ID: 36115835

Experiment series 3

Protein A

Protein: DLG2
Fragment: 189-283
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: RASSF6
Fragment: 360-369
Site: PBM
Sequence: KLVIKTETTV
Construct: biotin-ttds-KLVIKTETTV
Average holdup BI: 0.35
Immobilized partner concentration (10-6M): 9
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
Measured pKd: 4.84
PUBMED ID: 36115835

Experiment series 4

Protein A

Protein: DLG2
Fragment: 418-513
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: RASSF6
Fragment: 360-369
Site: PBM
Sequence: KLVIKTETTV
Construct: biotin-ttds-KLVIKTETTV
Average holdup BI: 0.21
Immobilized partner concentration (10-6M): 9
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
Measured pKd: 4.51
PUBMED ID: 36115835

Experiment series 5

Protein A

Protein: DLG2
Fragment: 418-513
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: RASSF6
Fragment: 360-369
Site: PBM
Sequence: KLVIKTETTV
Construct: biotin-ttds-KLVIKTETTV
Average holdup BI: 0.37
Normalized holdup BI: 0.37
Immobilized partner concentration (10-6M): 20.7
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.49
PUBMED ID: 36115835