Interaction Details


Details of experiments between Q15700 and P60484
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: DLG2
Fragment: 189-283
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: PTEN
Fragment: 393-403
Site: PBM
Sequence: EDQHTQITKV
Construct: biotin-ttds-DEDQHTQITKV
Average holdup BI: 0.17
Immobilized partner concentration (10-6M): 36
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 1
Measured pKd: 3.76
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: DLG2
Fragment: 189-283
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: PTEN
Fragment: 394-403
Site: PBM
Sequence: EDQHTQITKV
Construct: Biotin-ado-ado-EDQHTQITKV
Average holdup BI: 0.05
Normalized holdup BI: 0.06
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.57
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6

Experiment series 3

Protein A

Protein: DLG2
Fragment: 189-283
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: PTEN
Fragment: 393-403
Site: PBM
Sequence: EDQHTQITKV
Construct: biotin-ttds-DEDQHTQITKV
Average holdup BI: 0.06
Normalized holdup BI: 0.06
Immobilized partner concentration (10-6M): 19
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.55
PUBMED ID: 36115835

Experiment series 4

Protein A

Protein: DLG2
Fragment: 189-283
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: PTEN
Fragment: 391-403
Site: PBM
Sequence: EDQHTQITKV
Construct: Biotin-ttds-PFDEDQHTQITKV
Average holdup BI: 0.05
Immobilized partner concentration (10-6M): 17
Experiment method: CALIP_holdup
Details of affinity fitting: lysozyme internal standard
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 33382810

Experiment series 5

Protein A

Protein: DLG2
Fragment: 418-513
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: PTEN
Fragment: 391-403
Site: PBM
Sequence: EDQHTQITKV
Construct: Biotin-ttds-PFDEDQHTQITKV
Average holdup BI: 0.08
Immobilized partner concentration (10-6M): 17
Experiment method: CALIP_holdup
Details of affinity fitting: lysozyme internal standard
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 33382810

Experiment series 6

Protein A

Protein: DLG2
Fragment: 418-513
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: PTEN
Fragment: 393-403
Site: PBM
Sequence: EDQHTQITKV
Construct: biotin-ttds-DEDQHTQITKV
Average holdup BI: 0.05
Normalized holdup BI: 0.05
Immobilized partner concentration (10-6M): 19
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 7

Protein A

Protein: DLG2
Fragment: 418-513
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: PTEN
Fragment: 394-403
Site: PBM
Sequence: EDQHTQITKV
Construct: Biotin-ado-ado-EDQHTQITKV
Average holdup BI: -0.02
Normalized holdup BI: -0.02
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6

Experiment series 8

Protein A

Protein: DLG2
Fragment: 93-186
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: PTEN
Fragment: 391-403
Site: PBM
Sequence: EDQHTQITKV
Construct: Biotin-ttds-PFDEDQHTQITKV
Average holdup BI: 0.04
Immobilized partner concentration (10-6M): 17
Experiment method: CALIP_holdup
Details of affinity fitting: lysozyme internal standard
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 33382810

Experiment series 9

Protein A

Protein: DLG2
Fragment: 93-186
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: PTEN
Fragment: 393-403
Site: PBM
Sequence: EDQHTQITKV
Construct: biotin-ttds-DEDQHTQITKV
Average holdup BI: 0.03
Immobilized partner concentration (10-6M): 36
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 10

Protein A

Protein: DLG2
Fragment: 418-513
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: PTEN
Fragment: 393-403
Site: PBM
Modification: (ALY)402
Sequence: EDQHTQITKV
Construct: biotin-ttds-DEDQHTQITKacV
Average holdup BI: 0.31
Normalized holdup BI: 0.31
Immobilized partner concentration (10-6M): 17
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.46
Experimental Details

not reported internal standards of holdup layout #5

Experiment series 11

Protein A

Protein: DLG2
Fragment: 189-283
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: PTEN
Fragment: 393-403
Site: PBM
Modification: (ALY)402
Sequence: EDQHTQITKV
Construct: biotin-ttds-DEDQHTQITKacV
Average holdup BI: 0.26
Normalized holdup BI: 0.31
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.42
PUBMED ID: 36115835
Experimental Details

not reported internal standards of holdup layout #6

Experiment series 12

Protein A

Protein: DLG2
Fragment: 189-283
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: PTEN
Fragment: 393-403
Site: PBM
Modification: (ALY)402
Sequence: EDQHTQITKV
Construct: biotin-ttds-DEDQHTQITKacV
Average holdup BI: 0.27
Normalized holdup BI: 0.27
Immobilized partner concentration (10-6M): 17
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.37
PUBMED ID: 36115835

Experiment series 13

Protein A

Protein: DLG2
Fragment: 189-283
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: PTEN
Fragment: 393-403
Site: PBM
Modification: (ALY)402
Sequence: EDQHTQITKV
Construct: biotin-ttds-DEDQHTQITKacV
Average holdup BI: 0.33
Immobilized partner concentration (10-6M): 29
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 1
Measured pKd: 4.25
PUBMED ID: 36115835

Experiment series 14

Protein A

Protein: DLG2
Fragment: 418-513
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: PTEN
Fragment: 393-403
Site: PBM
Modification: (ALY)402
Sequence: EDQHTQITKV
Construct: biotin-ttds-DEDQHTQITKacV
Average holdup BI: 0.16
Normalized holdup BI: 0.16
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.03
PUBMED ID: 36115835
Experimental Details

not reported internal standards of holdup layout #6

Experiment series 15

Protein A

Protein: DLG2
Fragment: 93-186
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: PTEN
Fragment: 393-403
Site: PBM
Modification: (ALY)402
Sequence: EDQHTQITKV
Construct: biotin-ttds-DEDQHTQITKacV
Average holdup BI: 0.21
Immobilized partner concentration (10-6M): 29
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 1
Measured pKd: 3.98
PUBMED ID: 36115835

Experiment series 16

Protein A

Protein: DLG2
Fragment: 189-283
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: PTEN
Fragment: 393-403
Site: PBM
Modification: (ALY)402
Sequence: EDQHTQITKV
Construct: biotin-ttds-DEDQHTQITKacV
Average holdup BI: 0.06
Normalized holdup BI: 0.07
Immobilized partner concentration (10-6M): 17
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.62
Experimental Details

not reported internal standards of holdup layout #5

Experiment series 17

Protein A

Protein: DLG2
Fragment: 418-513
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: PTEN
Fragment: 393-403
Site: PBM
Modification: (ALY)402
Sequence: EDQHTQITKV
Construct: biotin-ttds-DEDQHTQITKacV
Average holdup BI: 0.05
Normalized holdup BI: 0.05
Immobilized partner concentration (10-6M): 17
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835