Interaction Details


Details of experiments between Q15700 and P17386
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: DLG2
Fragment: 189-283
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV31-E6
Fragment: 140-149
Site: PBM
Sequence: SRRPRTETQV
Construct: biotin-ttds-SRRPRTETQV
Average holdup BI: 0.78
Holdup BI standard deviation: 0
Normalized holdup BI: 0.78
Normalized holdup BI standard deviation: 0
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 2
Measured pKd: 5.37
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: DLG2
Fragment: 418-513
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV31-E6
Fragment: 140-149
Site: PBM
Sequence: SRRPRTETQV
Construct: biotin-ttds-SRRPRTETQV
Average holdup BI: 0.66
Holdup BI standard deviation: 0
Normalized holdup BI: 0.66
Normalized holdup BI standard deviation: 0
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 2
Measured pKd: 5.1
PUBMED ID: 36115835