Interaction Details


Details of experiments between Q15617 and P78352
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: OR8G1
Fragment: 302-311
Site: PBM
Sequence: KKMLQRRTLL
Construct: biotin-ado-ado-KKMLQRRTLL

Protein B

Protein: DLG4
Fragment: 62-152
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.1
Normalized holdup BI: 0.11
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.87
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.

Experiment series 2

Protein A

Protein: OR8G1
Fragment: 302-311
Site: PBM
Sequence: KKMLQRRTLL
Construct: biotin-ado-ado-KKMLQRRTLL

Protein B

Protein: DLG4
Fragment: 155-249
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.08
Normalized holdup BI: 0.1
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.81
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.

Experiment series 3

Protein A

Protein: OR8G1
Fragment: 302-311
Site: PBM
Sequence: KKMLQRRTLL
Construct: biotin-ado-ado-KKMLQRRTLL

Protein B

Protein: DLG4
Fragment: 306-403
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.03
Normalized holdup BI: 0.04
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.