Interaction Details


Details of experiments between Q15599 and Q9Y6N9
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: NHERF2
Fragment: 142-238
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: USH1C
Fragment: 543-552
Site: PBM
Sequence: KEYDDELTFF
Construct: Biotin-Ahx-KEYDDELTFF
Average holdup BI: 0.76
Normalized holdup BI: 0.76
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 5.32
PUBMED ID: 36115835