Interaction Details


Details of experiments between Q15599 and Q9Y2H0
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: NHERF2
Fragment: 142-238
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: DLGAP4
Fragment: 983-992
Site: PBM
Sequence: IYVPEAQTRL
Construct: biotin-ttds-IYVPEAQTRL
Average holdup BI: 0.52
Normalized holdup BI: 0.52
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.84
PUBMED ID: 36115835