Interaction Details

Details of experiments between Q15599 and Q3I5F7
Note that the results of all experiments are listed, regardless of the modification states of the fragments.

Experiment series 1

Protein A

Protein: NHERF2
Fragment: 142-238
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: ACOT6
Fragment: 198-207
Site: PBM
Sequence: GKKSVKHSKI
Construct: biotin-ado-ado-GKKSVKHSKI

Average holdup BI: -0.02

Normalized holdup BI: -0.02

Immobilized partner concentration (10^-6M): 18

Experiment method: DAPF_holdup

Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout

Number of measurements: 1

The affinity was below the detection threshold of the assay.

PUBMED ID: 36115835

Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.