Interaction Details

Details of experiments between Q15599 and O14936
Note that the results of all experiments are listed, regardless of the modification states of the fragments.

Experiment series 1

Protein A

Protein: NHERF2
Fragment: 328-337
Site: PBM
Sequence: NRKREIFSNF
Construct: Biotin-Ahx-NRKREIFSNF

Protein B

Protein: CASK
Fragment: 487-570
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Average holdup BI: -0.04

Normalized holdup BI: -0.04

Immobilized partner concentration (10^-6M): 18

Experiment method: DAPF_holdup

Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout

Number of measurements: 1

The affinity was below the detection threshold of the assay.

PUBMED ID: 36115835

Experimental Details

The peptide stock solution had solubility issues. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.