Interaction Details


Details of experiments between Q15418 and Q9H5P4
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: RPS6KA1
Fragment: 729-735
Site: PBM
Sequence: RVRKLPSTTL
Construct: Fluorescein-KLPSTTL

Protein B

Protein: PDZD7
Fragment: 858-953
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ
Measured dissociation constant (10-6M): 0.8
Standard deviation of dissociation constant (10-6M): 0.05
Experiment method: FP_direct
Number of measurements: 3
Measured pKd: 6.1
PUBMED ID: 30726710

Experiment series 2

Protein A

Protein: RPS6KA1
Fragment: 683-735
Site: PBM
Sequence: RVRKLPSTTL
Construct: ...KLPSTTL

Protein B

Protein: PDZD7
Fragment: 858-953
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ
Measured dissociation constant (10-6M): 4
Standard deviation of dissociation constant (10-6M): 1
Experiment method: FP_competitive
Number of measurements: 3
Measured pKd: 5.4
PUBMED ID: 30726710

Experiment series 3

Protein A

Protein: RPS6KA1
Fragment: 725-735
Site: PBM
Sequence: RVRKLPSTTL
Construct: Biotin-ttds-RRVRKLPSTTL

Protein B

Protein: PDZD7
Fragment: 858-953
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ
Measured dissociation constant (10-6M): 6.2
Standard deviation of dissociation constant (10-6M): 0.9
Experiment method: SPR
Number of measurements: 1
Measured pKd: 5.21
PUBMED ID: 30726710

Experiment series 4

Protein A

Protein: RPS6KA1
Fragment: 726-735
Site: PBM
Sequence: RVRKLPSTTL
Construct: Biotin-ado-ado-RVRKLPSTTL

Protein B

Protein: PDZD7
Fragment: 858-953
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.6
Normalized holdup BI: 0.63
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 5.04
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6

Experiment series 5

Protein A

Protein: RPS6KA1
Fragment: 725-735
Site: PBM
Sequence: RVRKLPSTTL
Construct: biotin-ttds-RRVRKLPSTTL

Protein B

Protein: PDZD7
Fragment: 858-953
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.46
Normalized holdup BI: 0.57
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.93
PUBMED ID: 36115835

Experiment series 6

Protein A

Protein: RPS6KA1
Fragment: 725-735
Site: PBM
Sequence: RVRKLPSTTL
Construct: biotin-ttds-RRVRKLPSTTL

Protein B

Protein: PDZD7
Fragment: 858-953
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ
Measured dissociation constant (10-6M): 11.9
Standard deviation of dissociation constant (10-6M): 2.31
Experiment method: competitive FP
Details of affinity fitting: Competitive binding equation in ProFit
Number of measurements: 3
Measured pKd: 4.92
PUBMED ID: 36115835

Experiment series 7

Protein A

Protein: RPS6KA1
Fragment: 725-735
Site: PBM
Sequence: RVRKLPSTTL
Construct: biotin-ttds-RRVRKLPSTTL

Protein B

Protein: PDZD7
Fragment: 858-953
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.64
Holdup BI standard deviation: 0.06
Immobilized partner concentration (10-6M): 31
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 5
Measured pKd: 4.79
PUBMED ID: 36115835

Experiment series 8

Protein A

Protein: RPS6KA1
Fragment: 725-735
Site: PBM
Sequence: RVRKLPSTTL
Construct: biotin-ttds-RRVRKLPSTTL

Protein B

Protein: PDZD7
Fragment: 199-313
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0
Holdup BI standard deviation: 0.11
Immobilized partner concentration (10-6M): 31
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 5
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 9

Protein A

Protein: RPS6KA1
Fragment: 725-735
Site: PBM
Sequence: RVRKLPSTTL
Construct: biotin-ttds-RRVRKLPSTTL

Protein B

Protein: PDZD7
Fragment: 82-170
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.02
Holdup BI standard deviation: 0.03
Immobilized partner concentration (10-6M): 31
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 4
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 10

Protein A

Protein: RPS6KA1
Fragment: 729-735
Site: PBM
Modification: (SEP)732
Sequence: RVRKLPSTTL
Construct: Fluorescein-KLP(pS)TTL

Protein B

Protein: PDZD7
Fragment: 858-953
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ
Measured dissociation constant (10-6M): 1.8
Standard deviation of dissociation constant (10-6M): 0.1
Experiment method: FP_direct
Number of measurements: 3
Measured pKd: 5.74
PUBMED ID: 30726710

Experiment series 11

Protein A

Protein: RPS6KA1
Fragment: 683-735
Site: PBM
Modification: (SEP)732
Sequence: RVRKLPSTTL
Construct: ...KLP(pS)TTL

Protein B

Protein: PDZD7
Fragment: 858-953
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ
Measured dissociation constant (10-6M): 46
Standard deviation of dissociation constant (10-6M): 7
Experiment method: FP_competitive
Number of measurements: 3
Measured pKd: 4.34
PUBMED ID: 30726710

Experiment series 12

Protein A

Protein: RPS6KA1
Fragment: 725-735
Site: PBM
Modification: (SEP)732
Sequence: RVRKLPSTTL
Construct: biotin-ttds-RRVRKLPpSTTL

Protein B

Protein: PDZD7
Fragment: 858-953
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.17
Normalized holdup BI: 0.2
Immobilized partner concentration (10-6M): 15
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.24
Experimental Details

not reported internal standards of holdup layout #5

Experiment series 13

Protein A

Protein: RPS6KA1
Fragment: 725-735
Site: PBM
Modification: (SEP)732
Sequence: RVRKLPSTTL
Construct: biotin-ttds-RRVRKLPpSTTL

Protein B

Protein: PDZD7
Fragment: 858-953
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ
Measured dissociation constant (10-6M): 99.41
Standard deviation of dissociation constant (10-6M): 16.92
Experiment method: competitive FP
Details of affinity fitting: Competitive binding equation in ProFit
Number of measurements: 3
Measured pKd: 4
PUBMED ID: 36115835

Experiment series 14

Protein A

Protein: RPS6KA1
Fragment: 725-735
Site: PBM
Modification: (SEP)732
Sequence: RVRKLPSTTL
Construct: biotin-ttds-RRVRKLPpSTTL

Protein B

Protein: PDZD7
Fragment: 858-953
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.06
Normalized holdup BI: 0.07
Immobilized partner concentration (10-6M): 15
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.72
PUBMED ID: 36115835

Experiment series 15

Protein A

Protein: RPS6KA1
Fragment: 725-735
Site: PBM
Modification: (SEP)732
Sequence: RVRKLPSTTL
Construct: biotin-ttds-RRVRKLPpSTTL

Protein B

Protein: PDZD7
Fragment: 858-953
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.13
Holdup BI standard deviation: 0.05
Immobilized partner concentration (10-6M): 40
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 4
Measured pKd: 3.56
PUBMED ID: 36115835

Experiment series 16

Protein A

Protein: RPS6KA1
Fragment: 725-735
Site: PBM
Modification: (SEP)732
Sequence: RVRKLPSTTL
Construct: biotin-ttds-RRVRKLPpSTTL

Protein B

Protein: PDZD7
Fragment: 199-313
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.11
Holdup BI standard deviation: 0.2
Immobilized partner concentration (10-6M): 40
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 4
Measured pKd: 3.49
PUBMED ID: 36115835

Experiment series 17

Protein A

Protein: RPS6KA1
Fragment: 725-735
Site: PBM
Modification: (SEP)732
Sequence: RVRKLPSTTL
Construct: biotin-ttds-RRVRKLPpSTTL

Protein B

Protein: PDZD7
Fragment: 82-170
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.09
Holdup BI standard deviation: 0.09
Immobilized partner concentration (10-6M): 40
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 3
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 18

Protein A

Protein: RPS6KA1
Fragment: 725-735
Site: PBM
Modification: (SEP)732
Sequence: RVRKLPSTTL
Construct: Biotin-ttds-RRVRKLP(pS)TTL

Protein B

Protein: PDZD7
Fragment: 858-953
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ
Measured dissociation constant (10-6M): 0
Experiment method: SPR
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 30726710

Experiment series 19

Protein A

Protein: RPS6KA1
Fragment: 725-735
Site: PBM
Modification: (TPO)733
Sequence: RVRKLPSTTL
Construct: biotin-ttds-RRVRKLPSpTTL

Protein B

Protein: PDZD7
Fragment: 858-953
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.04
Normalized holdup BI: 0.05
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.5
PUBMED ID: 36115835

Experiment series 20

Protein A

Protein: RPS6KA1
Fragment: 725-735
Site: PBM
Modification: (TPO)733
Sequence: RVRKLPSTTL
Construct: biotin-ttds-RRVRKLPSpTTL

Protein B

Protein: PDZD7
Fragment: 858-953
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ
Measured dissociation constant (10-6M): 0
Experiment method: competitive FP
Details of affinity fitting: Competitive binding equation in ProFit
Number of measurements: 3
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 21

Protein A

Protein: RPS6KA1
Fragment: 725-735
Site: PBM
Modification: (TPO)734
Sequence: RVRKLPSTTL
Construct: Biotin-ttds-RRVRKLPSTpTL

Protein B

Protein: PDZD7
Fragment: 858-953
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.54
Normalized holdup BI: 0.65
Immobilized partner concentration (10-6M): 25
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.91
PUBMED ID: 36115835

Experiment series 22

Protein A

Protein: RPS6KA1
Fragment: 725-735
Site: PBM
Modification: (TPO)734
Sequence: RVRKLPSTTL
Construct: Biotin-ttds-RRVRKLPSTpTL

Protein B

Protein: PDZD7
Fragment: 858-953
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ
Measured dissociation constant (10-6M): 12.7
Standard deviation of dissociation constant (10-6M): 2.52
Experiment method: competitive FP
Details of affinity fitting: Competitive binding equation in ProFit
Number of measurements: 3
Measured pKd: 4.9
PUBMED ID: 36115835