Interaction Details

Details of experiments between Q15311 and Q9C0E4
Note that the results of all experiments are listed, regardless of the modification states of the fragments.

Experiment series 1

Protein A

Protein: RALBP1
Fragment: 646-655
Site: PBM
Sequence: PSRDRKETSI
Construct: biotin-ttds-PSRDRKETSI

Protein B

Protein: GRIP2
Fragment: 137-245
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Average holdup BI: 0.25

Immobilized partner concentration (10^-6M): 20

Experiment method: SAPF_holdup

Details of affinity fitting: fluorescein + mCherry internal standards + normal layout

Number of measurements: 1

Measured pK_d: 4.25

PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: RALBP1
Fragment: 646-655
Site: PBM
Sequence: PSRDRKETSI
Construct: biotin-ttds-PSRDRKETSI

Protein B

Protein: GRIP2
Fragment: 238-339
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ

Average holdup BI: 0.12

Immobilized partner concentration (10^-6M): 20

Experiment method: SAPF_holdup

Details of affinity fitting: fluorescein + mCherry internal standards + normal layout

Number of measurements: 1

Measured pK_d: 3.85

PUBMED ID: 36115835

Experiment series 3

Protein A

Protein: RALBP1
Fragment: 646-655
Site: PBM
Sequence: PSRDRKETSI
Construct: biotin-ttds-PSRDRKETSI

Protein B

Protein: GRIP2
Fragment: 649-740
Site: PDZ_6
Construct: his6-MBP-TEVsite-PDZ

Average holdup BI: 0.06

Normalized holdup BI: 0.06

Immobilized partner concentration (10^-6M): 28

Experiment method: DAPF_holdup

Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout

Number of measurements: 1

Measured pK_d: 3.36

PUBMED ID: 36115835

Experiment series 4

Protein A

Protein: RALBP1
Fragment: 646-655
Site: PBM
Sequence: PSRDRKETSI
Construct: biotin-ttds-PSRDRKETSI

Protein B

Protein: GRIP2
Fragment: 238-339
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ

Average holdup BI: -0.03

Normalized holdup BI: -0.03

Immobilized partner concentration (10^-6M): 18

Experiment method: DAPF_holdup

Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout

Number of measurements: 1

The affinity was below the detection threshold of the assay.

PUBMED ID: 36115835

Experimental Details

not reported internal standards of holdup layout #6

Experiment series 5

Protein A

Protein: RALBP1
Fragment: 646-655
Site: PBM
Sequence: PSRDRKETSI
Construct: biotin-ttds-PSRDRKETSI

Protein B

Protein: GRIP2
Fragment: 238-339
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ

Average holdup BI: 0

Normalized holdup BI: 0

Immobilized partner concentration (10^-6M): 28

Experiment method: DAPF_holdup

Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout

Number of measurements: 1

The affinity was below the detection threshold of the assay.

PUBMED ID: 36115835

Experiment series 6

Protein A

Protein: RALBP1
Fragment: 646-655
Site: PBM
Sequence: PSRDRKETSI
Construct: biotin-ttds-PSRDRKETSI

Protein B

Protein: GRIP2
Fragment: 41-133
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Average holdup BI: 0.01

Immobilized partner concentration (10^-6M): 20

Experiment method: SAPF_holdup

Details of affinity fitting: fluorescein + mCherry internal standards + normal layout

Number of measurements: 1

The affinity was below the detection threshold of the assay.

PUBMED ID: 36115835

Experiment series 7

Protein A

Protein: RALBP1
Fragment: 646-655
Site: PBM
Sequence: PSRDRKETSI
Construct: biotin-ttds-PSRDRKETSI

Protein B

Protein: GRIP2
Fragment: 446-548
Site: PDZ_4
Construct: his6-MBP-TEVsite-PDZ

Average holdup BI: -0.04

Normalized holdup BI: -0.04

Immobilized partner concentration (10^-6M): 18

Experiment method: DAPF_holdup

Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout

Number of measurements: 1

The affinity was below the detection threshold of the assay.

PUBMED ID: 36115835

Experimental Details

not reported internal standards of holdup layout #6

Experiment series 8

Protein A

Protein: RALBP1
Fragment: 646-655
Site: PBM
Sequence: PSRDRKETSI
Construct: biotin-ttds-PSRDRKETSI

Protein B

Protein: GRIP2
Fragment: 446-548
Site: PDZ_4
Construct: his6-MBP-TEVsite-PDZ

Average holdup BI: 0

Normalized holdup BI: 0

Immobilized partner concentration (10^-6M): 28

Experiment method: DAPF_holdup

Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout

Number of measurements: 1

The affinity was below the detection threshold of the assay.

PUBMED ID: 36115835

Experiment series 9

Protein A

Protein: RALBP1
Fragment: 646-655
Site: PBM
Sequence: PSRDRKETSI
Construct: biotin-ttds-PSRDRKETSI

Protein B

Protein: GRIP2
Fragment: 446-548
Site: PDZ_4
Construct: his6-MBP-TEVsite-PDZ

Average holdup BI: 0.03

Immobilized partner concentration (10^-6M): 20

Experiment method: SAPF_holdup

Details of affinity fitting: fluorescein + mCherry internal standards + normal layout

Number of measurements: 1

The affinity was below the detection threshold of the assay.

PUBMED ID: 36115835

Experiment series 10

Protein A

Protein: RALBP1
Fragment: 646-655
Site: PBM
Sequence: PSRDRKETSI
Construct: biotin-ttds-PSRDRKETSI

Protein B

Protein: GRIP2
Fragment: 544-642
Site: PDZ_5
Construct: his6-MBP-TEVsite-PDZ

Average holdup BI: -0.03

Normalized holdup BI: -0.03

Immobilized partner concentration (10^-6M): 18

Experiment method: DAPF_holdup

Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout

Number of measurements: 1

The affinity was below the detection threshold of the assay.

PUBMED ID: 36115835

Experimental Details

not reported internal standards of holdup layout #6

Experiment series 11

Protein A

Protein: RALBP1
Fragment: 646-655
Site: PBM
Sequence: PSRDRKETSI
Construct: biotin-ttds-PSRDRKETSI

Protein B

Protein: GRIP2
Fragment: 544-642
Site: PDZ_5
Construct: his6-MBP-TEVsite-PDZ

Average holdup BI: 0.01

Normalized holdup BI: 0.01

Immobilized partner concentration (10^-6M): 28

Experiment method: DAPF_holdup

Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout

Number of measurements: 1

The affinity was below the detection threshold of the assay.

PUBMED ID: 36115835

Experiment series 12

Protein A

Protein: RALBP1
Fragment: 646-655
Site: PBM
Sequence: PSRDRKETSI
Construct: biotin-ttds-PSRDRKETSI

Protein B

Protein: GRIP2
Fragment: 544-642
Site: PDZ_5
Construct: his6-MBP-TEVsite-PDZ

Average holdup BI: 0.05

Immobilized partner concentration (10^-6M): 20

Experiment method: SAPF_holdup

Details of affinity fitting: fluorescein + mCherry internal standards + normal layout

Number of measurements: 1

The affinity was below the detection threshold of the assay.

PUBMED ID: 36115835

Experiment series 13

Protein A

Protein: RALBP1
Fragment: 646-655
Site: PBM
Sequence: PSRDRKETSI
Construct: biotin-ttds-PSRDRKETSI

Protein B

Protein: GRIP2
Fragment: 649-740
Site: PDZ_6
Construct: his6-MBP-TEVsite-PDZ

Average holdup BI: -0.01

Normalized holdup BI: -0.01

Immobilized partner concentration (10^-6M): 18

Experiment method: DAPF_holdup

Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout

Number of measurements: 1

The affinity was below the detection threshold of the assay.

PUBMED ID: 36115835

Experimental Details

not reported internal standards of holdup layout #6

Experiment series 14

Protein A

Protein: RALBP1
Fragment: 646-655
Site: PBM
Sequence: PSRDRKETSI
Construct: biotin-ttds-PSRDRKETSI

Protein B

Protein: GRIP2
Fragment: 649-740
Site: PDZ_6
Construct: his6-MBP-TEVsite-PDZ

Average holdup BI: 0

Immobilized partner concentration (10^-6M): 20

Experiment method: SAPF_holdup

Details of affinity fitting: fluorescein + mCherry internal standards + normal layout

Number of measurements: 1

The affinity was below the detection threshold of the assay.

PUBMED ID: 36115835

Experiment series 15

Protein A

Protein: RALBP1
Fragment: 646-655
Site: PBM
Sequence: PSRDRKETSI
Construct: biotin-ttds-PSRDRKETSI

Protein B

Protein: GRIP2
Fragment: 934-1023
Site: PDZ_7
Construct: his6-MBP-TEVsite-PDZ

Average holdup BI: -0.16

Normalized holdup BI: -0.16

Immobilized partner concentration (10^-6M): 28

Experiment method: DAPF_holdup

Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout

Number of measurements: 1

The affinity was below the detection threshold of the assay.

PUBMED ID: 36115835

Experiment series 16

Protein A

Protein: RALBP1
Fragment: 646-655
Site: PBM
Sequence: PSRDRKETSI
Construct: biotin-ttds-PSRDRKETSI

Protein B

Protein: GRIP2
Fragment: 934-1023
Site: PDZ_7
Construct: his6-MBP-TEVsite-PDZ

Average holdup BI: -0.03

Immobilized partner concentration (10^-6M): 20

Experiment method: SAPF_holdup

Details of affinity fitting: fluorescein + mCherry internal standards + normal layout

Number of measurements: 1

The affinity was below the detection threshold of the assay.

PUBMED ID: 36115835

Experiment series 17

Protein A

Protein: RALBP1
Fragment: 646-655
Site: PBM
Sequence: PSRDRKETSI
Construct: biotin-ttds-PSRDRKETSI

Protein B

Protein: GRIP2
Fragment: 934-1023
Site: PDZ_7
Construct: his6-MBP-TEVsite-PDZ

Average holdup BI: 0

Normalized holdup BI: 0

Immobilized partner concentration (10^-6M): 18

Experiment method: DAPF_holdup

Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout

Number of measurements: 1

The affinity was below the detection threshold of the assay.

PUBMED ID: 36115835

Experimental Details

not reported internal standards of holdup layout #6