Interaction Details


Details of experiments between Q15276 and Q04917
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: RABEP1
Fragment: 157-166
Site: 14-3-3_pSer162
Modification: (SEP)162
Sequence: LRRRLSEGQE
Construct: LRRRL(pSer)EGQE (crude synthetic peptide)

Protein B

Protein: YWHAH
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3eta
Average holdup BI: -0.02
Holdup BI standard deviation: 0.39
Immobilized partner concentration (10-6M): 130
P value: 0.03
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 2

Protein A

Protein: RABEP1
Fragment: 372-381
Site: 14-3-3_pSer377
Modification: (SEP)377
Sequence: RGSVHSLDAG
Construct: RGSVH(pSer)LDAG (crude synthetic peptide)

Protein B

Protein: YWHAH
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3eta
Average holdup BI: 0.38
Holdup BI standard deviation: 0.05
Immobilized partner concentration (10-6M): 130
P value: 5.74
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 3.67
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 3

Protein A

Protein: RABEP1
Fragment: 402-411
Site: 14-3-3_pSer407
Modification: (SEP)407
Sequence: LRRAQSTDSL
Construct: LRRAQ(pSer)TDSL (crude synthetic peptide)

Protein B

Protein: YWHAH
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3eta
Average holdup BI: 0.69
Holdup BI standard deviation: 0.07
Immobilized partner concentration (10-6M): 130
P value: 6.63
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 4.24
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 4

Protein A

Protein: RABEP1
Fragment: 411-420
Site: 14-3-3_pSer416
Modification: (SEP)416
Sequence: LGTSGSLQSK
Construct: LGTSG(pSer)LQSK (crude synthetic peptide)

Protein B

Protein: YWHAH
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3eta
Average holdup BI: 0.22
Holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 130
P value: 6.53
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 3.33
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 5

Protein A

Protein: RABEP1
Fragment: 425-434
Site: 14-3-3_pSer430
Modification: (SEP)430
Sequence: NYKAKSAGNL
Construct: NYKAK(pSer)AGNL (crude synthetic peptide)

Protein B

Protein: YWHAH
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3eta
Average holdup BI: 0.88
Holdup BI standard deviation: 0.08
Immobilized partner concentration (10-6M): 130
P value: 5.61
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 4.74
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 6

Protein A

Protein: RABEP1
Fragment: 408-417
Site: 14-3-3_pThr413
Modification: (TPO)413
Sequence: TDSLGTSGSL
Construct: TDSLG(pThr)SGSL (crude synthetic peptide)

Protein B

Protein: YWHAH
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3eta
Average holdup BI: -0.14
Holdup BI standard deviation: 0.14
Immobilized partner concentration (10-6M): 130
P value: 1.1
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))