Interaction Details


Details of experiments between Q15018 and Q9NY99
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: ABRAXAS2
Fragment: 406-415
Site: PBM
Sequence: DPRNTQTSQI
Construct: biotin-ttds-DPRNTQTSQI

Protein B

Protein: SNTG2
Fragment: 70-159
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.07
Holdup BI standard deviation: 0.02
Normalized holdup BI: 0.08
Normalized holdup BI standard deviation: 0.03
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 2
Measured pKd: 3.7
PUBMED ID: 36115835