Interaction Details


Details of experiments between Q15018 and Q12959
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: ABRAXAS2
Fragment: 1-415
Construct: endogenous protein (Jurkat)

Protein B

Protein: DLG1
Fragment: 314-412
Site: PDZ_2
Construct: avi(biotin)-his6-MBP-TEVsite-PDZ
Average holdup BI: 0.4
Immobilized partner concentration (10-6M): 10
P value: 5.88
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-08-03
Number of measurements: 3
Measured pKd: 4.83
PUBMED ID: 36542723
Experimental Details

-log10(P Two-sided unpaired T-test (6 vs 3))

Experiment series 2

Protein A

Protein: ABRAXAS2
Fragment: 406-415
Site: PBM
Sequence: DPRNTQTSQI
Construct: biotin-ttds-DPRNTQTSQI

Protein B

Protein: DLG1
Fragment: 314-412
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.1
Normalized holdup BI: 0.11
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.84
PUBMED ID: 36115835

Experiment series 3

Protein A

Protein: ABRAXAS2
Fragment: 406-415
Site: PBM
Sequence: DPRNTQTSQI
Construct: biotin-ttds-DPRNTQTSQI

Protein B

Protein: DLG1
Fragment: 457-570
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.05
Normalized holdup BI: 0.06
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.52
PUBMED ID: 36115835

Experiment series 4

Protein A

Protein: ABRAXAS2
Fragment: 406-415
Site: PBM
Sequence: DPRNTQTSQI
Construct: biotin-ttds-DPRNTQTSQI

Protein B

Protein: DLG1
Fragment: 221-314
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.04
Normalized holdup BI: 0.05
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.49
PUBMED ID: 36115835

Experiment series 5

Protein A

Protein: ABRAXAS2
Fragment: 1-415
Construct: endogenous protein (Jurkat)

Protein B

Protein: DLG1
Fragment: 221-314
Site: PDZ_1
Construct: avi(biotin)-his6-MBP-TEVsite-PDZ
Average holdup BI: 0.07
Immobilized partner concentration (10-6M): 10
P value: 0.68
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-08-03
Number of measurements: 3
The affinity was below the detection threshold of the assay.
PUBMED ID: 36542723
Experimental Details

-log10(P Two-sided unpaired T-test (6 vs 3))