Interaction Details


Details of experiments between Q14CM0 and Q6UVM3
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: FRMPD4
Fragment: 67-160
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: KCNT2
Fragment: 1126-1135
Site: PBM
Sequence: GQDSREETQL
Construct: biotin-ttds-GQDSREETQL
Average holdup BI: 0.22
Normalized holdup BI: 0.27
Immobilized partner concentration (10-6M): 13.3
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.48
PUBMED ID: 36115835