Interaction Details


Details of experiments between Q14CM0 and Q15700
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: FRMPD4
Fragment: 1313-1322
Site: PBM
Sequence: RLPKIKETTV
Construct: Biotin-ado-ado-RLPKIKETTV

Protein B

Protein: DLG2
Fragment: 189-283
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.81
Normalized holdup BI: 0.95
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 6.14
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6

Experiment series 2

Protein A

Protein: FRMPD4
Fragment: 1313-1322
Site: PBM
Sequence: RLPKIKETTV
Construct: biotin-ttds-RLPKIKETTV

Protein B

Protein: DLG2
Fragment: 189-283
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.85
Holdup BI standard deviation: 0.05
Normalized holdup BI: 0.85
Normalized holdup BI standard deviation: 0.05
Immobilized partner concentration (10-6M): 21.6
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 2
Measured pKd: 5.5
PUBMED ID: 36115835

Experiment series 3

Protein A

Protein: FRMPD4
Fragment: 1313-1322
Site: PBM
Sequence: RLPKIKETTV
Construct: biotin-ttds-RLPKIKETTV

Protein B

Protein: DLG2
Fragment: 93-186
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.49
Immobilized partner concentration (10-6M): 6.6
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
Measured pKd: 5.32
PUBMED ID: 36115835

Experiment series 4

Protein A

Protein: FRMPD4
Fragment: 1313-1322
Site: PBM
Sequence: RLPKIKETTV
Construct: Biotin-ado-ado-RLPKIKETTV

Protein B

Protein: DLG2
Fragment: 418-513
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.74
Normalized holdup BI: 0.74
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 5.29
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6

Experiment series 5

Protein A

Protein: FRMPD4
Fragment: 1313-1322
Site: PBM
Sequence: RLPKIKETTV
Construct: biotin-ttds-RLPKIKETTV

Protein B

Protein: DLG2
Fragment: 418-513
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.73
Holdup BI standard deviation: 0.05
Normalized holdup BI: 0.73
Normalized holdup BI standard deviation: 0.05
Immobilized partner concentration (10-6M): 21.6
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 2
Measured pKd: 5.15
PUBMED ID: 36115835

Experiment series 6

Protein A

Protein: FRMPD4
Fragment: 1313-1322
Site: PBM
Sequence: RLPKIKETTV
Construct: biotin-ttds-RLPKIKETTV

Protein B

Protein: DLG2
Fragment: 189-283
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.39
Immobilized partner concentration (10-6M): 6.6
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
Measured pKd: 5.11
PUBMED ID: 36115835

Experiment series 7

Protein A

Protein: FRMPD4
Fragment: 1313-1322
Site: PBM
Sequence: RLPKIKETTV
Construct: biotin-ttds-RLPKIKETTV

Protein B

Protein: DLG2
Fragment: 418-513
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.25
Immobilized partner concentration (10-6M): 6.6
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
Measured pKd: 4.77
PUBMED ID: 36115835