Interaction Details


Details of experiments between Q14CM0 and Q15418
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: FRMPD4
Fragment: 67-160
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: RPS6KA1
Fragment: 726-735
Site: PBM
Sequence: RVRKLPSTTL
Construct: Biotin-ado-ado-RVRKLPSTTL
Average holdup BI: 0.07
Normalized holdup BI: 0.07
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.64
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6

Experiment series 2

Protein A

Protein: FRMPD4
Fragment: 67-160
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: RPS6KA1
Fragment: 725-735
Site: PBM
Sequence: RVRKLPSTTL
Construct: biotin-ttds-RRVRKLPSTTL
Average holdup BI: 0.03
Holdup BI standard deviation: 0
Normalized holdup BI: 0.03
Normalized holdup BI standard deviation: 0
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 3

Protein A

Protein: FRMPD4
Fragment: 67-160
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: RPS6KA1
Fragment: 725-735
Site: PBM
Sequence: RVRKLPSTTL
Construct: biotin-ttds-RRVRKLPSTTL
Average holdup BI: 0.06
Holdup BI standard deviation: 0.07
Immobilized partner concentration (10-6M): 31
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 4
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 4

Protein A

Protein: FRMPD4
Fragment: 67-160
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: RPS6KA1
Fragment: 725-735
Site: PBM
Modification: (SEP)732
Sequence: RVRKLPSTTL
Construct: biotin-ttds-RRVRKLPpSTTL
Average holdup BI: 0.15
Normalized holdup BI: 0.17
Immobilized partner concentration (10-6M): 15
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.17
Experimental Details

not reported internal standards of holdup layout #5

Experiment series 5

Protein A

Protein: FRMPD4
Fragment: 67-160
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: RPS6KA1
Fragment: 725-735
Site: PBM
Modification: (SEP)732
Sequence: RVRKLPSTTL
Construct: biotin-ttds-RRVRKLPpSTTL
Average holdup BI: 0.13
Normalized holdup BI: 0.14
Immobilized partner concentration (10-6M): 15
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.04
Experimental Details

not reported internal standards of holdup layout #5

Experiment series 6

Protein A

Protein: FRMPD4
Fragment: 67-160
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: RPS6KA1
Fragment: 725-735
Site: PBM
Modification: (SEP)732
Sequence: RVRKLPSTTL
Construct: biotin-ttds-RRVRKLPpSTTL
Average holdup BI: 0.06
Holdup BI standard deviation: 0
Normalized holdup BI: 0.07
Normalized holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 15
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 2
Measured pKd: 3.73
PUBMED ID: 36115835

Experiment series 7

Protein A

Protein: FRMPD4
Fragment: 67-160
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: RPS6KA1
Fragment: 725-735
Site: PBM
Modification: (SEP)732
Sequence: RVRKLPSTTL
Construct: biotin-ttds-RRVRKLPpSTTL
Average holdup BI: 0.06
Holdup BI standard deviation: 0.08
Immobilized partner concentration (10-6M): 40
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 3
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 8

Protein A

Protein: FRMPD4
Fragment: 67-160
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: RPS6KA1
Fragment: 725-735
Site: PBM
Modification: (TPO)733
Sequence: RVRKLPSTTL
Construct: biotin-ttds-RRVRKLPSpTTL
Average holdup BI: 0.04
Holdup BI standard deviation: 0
Normalized holdup BI: 0.05
Normalized holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 2
Measured pKd: 3.48
PUBMED ID: 36115835

Experiment series 9

Protein A

Protein: FRMPD4
Fragment: 67-160
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: RPS6KA1
Fragment: 725-735
Site: PBM
Modification: (TPO)734
Sequence: RVRKLPSTTL
Construct: Biotin-ttds-RRVRKLPSTpTL
Average holdup BI: 0.08
Holdup BI standard deviation: 0.02
Normalized holdup BI: 0.09
Normalized holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 25
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 2
Measured pKd: 3.62
PUBMED ID: 36115835