Interaction Details


Details of experiments between Q14CM0 and P0DTC4
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: FRMPD4
Fragment: 67-160
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: SARS-CoV2-E
Fragment: 66-75
Site: PBM
Sequence: NSSRVPDLLV
Construct: biotin-NLNSSRVPDLLV
Average holdup BI: 0.18
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 20
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 2
Measured pKd: 4.06
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: FRMPD4
Fragment: 67-160
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: SARS-CoV2-E
Fragment: 66-75
Site: PBM
Sequence: NSSRVPDLLV
Construct: biotin-NLNSSRVPDLLV
Average holdup BI: 0.04
Normalized holdup BI: 0.05
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.48
PUBMED ID: 36115835