Interaction Details


Details of experiments between Q14CM0 and O15439
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: FRMPD4
Fragment: 67-160
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: ABCC4
Fragment: 1316-1325
Site: PBM
Sequence: STLTIFETAL
Construct: biotin-ttds-STLTIFETAL
Average holdup BI: 0.36
Immobilized partner concentration (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
Measured pKd: 4.82
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: FRMPD4
Fragment: 67-160
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: ABCC4
Fragment: 1316-1325
Site: PBM
Sequence: STLTIFETAL
Construct: Biotin-ado-ado-STLTIFETAL
Average holdup BI: 0.51
Normalized holdup BI: 0.51
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.81
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6

Experiment series 3

Protein A

Protein: FRMPD4
Fragment: 67-160
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: ABCC4
Fragment: 1316-1325
Site: PBM
Sequence: STLTIFETAL
Construct: biotin-ttds-STLTIFETAL
Average holdup BI: 0.37
Normalized holdup BI: 0.42
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.65
Experimental Details

not reported internal standards of holdup layout #5

Experiment series 4

Protein A

Protein: FRMPD4
Fragment: 67-160
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: ABCC4
Fragment: 1316-1325
Site: PBM
Sequence: STLTIFETAL
Construct: biotin-ttds-STLTIFETAL
Average holdup BI: 0.33
Holdup BI standard deviation: 0.02
Normalized holdup BI: 0.41
Normalized holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 3
Measured pKd: 4.64
PUBMED ID: 36115835

Experiment series 5

Protein A

Protein: FRMPD4
Fragment: 67-160
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: ABCC4
Fragment: 1316-1325
Site: PBM
Sequence: STLTIFETAL
Construct: biotin-ttds-STLTIFETAL
Average holdup BI: 0.34
Normalized holdup BI: 0.37
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.55
Experimental Details

not reported internal standards of holdup layout #5