Interaction Details


Details of experiments between Q14674 and O00499
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: ESPL1
Fragment: 1-2120
Construct: endogenous protein (Jurkat)

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3
Average holdup BI: 0.72
Immobilized partner concentration (10-6M): 10
P value: 5.66
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-06-29
Number of measurements: 3
Measured pKd: 5.41
PUBMED ID: 38995680
Experimental Details

-log10(P Two-sided unpaired T-test (6 vs 3))

Experiment series 2

Protein A

Protein: ESPL1
Fragment: 1341-1355
Site: PxxP_2
Sequence: RGLPCTPKPPDRIRQ
Construct: Bio Ado Ado-RGLPCTPKPPDRIRQ-CONH2

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.38
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
Measured pKd: 4.58
PUBMED ID: 38995680

Experiment series 3

Protein A

Protein: ESPL1
Fragment: 1338-1352
Site: PxxP_1
Sequence: LEGRGLPCTPKPPDR
Construct: Bio Ado Ado-LEGRGLPCTPKPPDR-CONH2

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.14
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
Measured pKd: 3.96
PUBMED ID: 38995680

Experiment series 4

Protein A

Protein: ESPL1
Fragment: 1338-1352
Site: PxxP_1
Sequence: LEGRGLPCTPKPPDR
Construct: Bio Ado Ado-LEGRGLPCTPKPPDR-CONH2

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: D537V
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: -0.03
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 5

Protein A

Protein: ESPL1
Fragment: 1341-1355
Site: PxxP_2
Sequence: RGLPCTPKPPDRIRQ
Construct: Bio Ado Ado-RGLPCTPKPPDRIRQ-CONH2

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: D537V
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 6

Protein A

Protein: ESPL1
Fragment: 1338-1352
Site: PxxP_1
Sequence: LEGRGLPCTPKPPDR
Construct: Bio Ado Ado-LEGRGLPCTPKPPDR-CONH2

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: F584S
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: -0.04
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 7

Protein A

Protein: ESPL1
Fragment: 1341-1355
Site: PxxP_2
Sequence: RGLPCTPKPPDRIRQ
Construct: Bio Ado Ado-RGLPCTPKPPDRIRQ-CONH2

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: F584S
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 8

Protein A

Protein: ESPL1
Fragment: 1341-1355
Site: PxxP_2
Sequence: RGLPCTPKPPDRIRQ
Construct: Bio Ado Ado-RGLPCTPKPPDRIRQ-CONH2

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: P551L
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.2
Immobilized partner concentration (10-6M): 8
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
Measured pKd: 4.54
PUBMED ID: 38995680

Experiment series 9

Protein A

Protein: ESPL1
Fragment: 1338-1352
Site: PxxP_1
Sequence: LEGRGLPCTPKPPDR
Construct: Bio Ado Ado-LEGRGLPCTPKPPDR-CONH2

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: P551L
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.03
Immobilized partner concentration (10-6M): 8
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 10

Protein A

Protein: ESPL1
Fragment: 1341-1355
Site: PxxP_2
Sequence: RGLPCTPKPPDRIRQ
Construct: Bio Ado Ado-RGLPCTPKPPDRIRQ-CONH2

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Q540H
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.34
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
Measured pKd: 4.49
PUBMED ID: 38995680

Experiment series 11

Protein A

Protein: ESPL1
Fragment: 1338-1352
Site: PxxP_1
Sequence: LEGRGLPCTPKPPDR
Construct: Bio Ado Ado-LEGRGLPCTPKPPDR-CONH2

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Q540H
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.14
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
Measured pKd: 3.96
PUBMED ID: 38995680

Experiment series 12

Protein A

Protein: ESPL1
Fragment: 1341-1355
Site: PxxP_2
Sequence: RGLPCTPKPPDRIRQ
Construct: Bio Ado Ado-RGLPCTPKPPDRIRQ-CONH2

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: R581C
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.21
Immobilized partner concentration (10-6M): 10
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
Measured pKd: 4.46
PUBMED ID: 38995680

Experiment series 13

Protein A

Protein: ESPL1
Fragment: 1338-1352
Site: PxxP_1
Sequence: LEGRGLPCTPKPPDR
Construct: Bio Ado Ado-LEGRGLPCTPKPPDR-CONH2

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: R581C
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.04
Immobilized partner concentration (10-6M): 10
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 14

Protein A

Protein: ESPL1
Fragment: 1341-1355
Site: PxxP_2
Sequence: RGLPCTPKPPDRIRQ
Construct: Bio Ado Ado-RGLPCTPKPPDRIRQ-CONH2

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: T532M
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.38
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
Measured pKd: 4.57
PUBMED ID: 38995680

Experiment series 15

Protein A

Protein: ESPL1
Fragment: 1338-1352
Site: PxxP_1
Sequence: LEGRGLPCTPKPPDR
Construct: Bio Ado Ado-LEGRGLPCTPKPPDR-CONH2

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: T532M
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.04
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 16

Protein A

Protein: ESPL1
Fragment: 1341-1355
Site: PxxP_2
Sequence: RGLPCTPKPPDRIRQ
Construct: Bio Ado Ado-RGLPCTPKPPDRIRQ-CONH2

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V566M
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.42
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
Measured pKd: 4.65
PUBMED ID: 38995680

Experiment series 17

Protein A

Protein: ESPL1
Fragment: 1338-1352
Site: PxxP_1
Sequence: LEGRGLPCTPKPPDR
Construct: Bio Ado Ado-LEGRGLPCTPKPPDR-CONH2

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V566M
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.1
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
Measured pKd: 3.81
PUBMED ID: 38995680

Experiment series 18

Protein A

Protein: ESPL1
Fragment: 1341-1355
Site: PxxP_2
Sequence: RGLPCTPKPPDRIRQ
Construct: Bio Ado Ado-RGLPCTPKPPDRIRQ-CONH2

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V583I
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.23
Immobilized partner concentration (10-6M): 13
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
Measured pKd: 4.4
PUBMED ID: 38995680

Experiment series 19

Protein A

Protein: ESPL1
Fragment: 1338-1352
Site: PxxP_1
Sequence: LEGRGLPCTPKPPDR
Construct: Bio Ado Ado-LEGRGLPCTPKPPDR-CONH2

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V583I
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: -0.01
Immobilized partner concentration (10-6M): 13
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 20

Protein A

Protein: ESPL1
Fragment: 1338-1352
Site: PxxP_1
Sequence: LEGRGLPCTPKPPDR
Construct: Bio Ado Ado-LEGRGLPCTPKPPDR-CONH2

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Y531S
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: -0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 21

Protein A

Protein: ESPL1
Fragment: 1341-1355
Site: PxxP_2
Sequence: RGLPCTPKPPDRIRQ
Construct: Bio Ado Ado-RGLPCTPKPPDRIRQ-CONH2

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Y531S
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.03
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680