Interaction Details


Details of experiments between Q14500 and Q15700
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: KCNJ12
Fragment: 424-433
Site: PBM
Sequence: QRPYRRESEI
Construct: biotin-ttds-QRPYRRESEI

Protein B

Protein: DLG2
Fragment: 418-513
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.42
Normalized holdup BI: 0.42
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.65
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: KCNJ12
Fragment: 424-433
Site: PBM
Sequence: QRPYRRESEI
Construct: biotin-ttds-QRPYRRESEI

Protein B

Protein: DLG2
Fragment: 189-283
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.14
Normalized holdup BI: 0.14
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.96
PUBMED ID: 36115835