Interaction Details


Details of experiments between Q14160 and Q6UVM3
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: SCRIB
Fragment: 999-1093
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: KCNT2
Fragment: 1126-1135
Site: PBM
Sequence: GQDSREETQL
Construct: biotin-ttds-GQDSREETQL
Average holdup BI: 0.51
Normalized holdup BI: 0.57
Immobilized partner concentration (10-6M): 13.3
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 5.07
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: SCRIB
Fragment: 718-814
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: KCNT2
Fragment: 1126-1135
Site: PBM
Sequence: GQDSREETQL
Construct: biotin-ttds-GQDSREETQL
Average holdup BI: 0.29
Holdup BI standard deviation: 0.06
Normalized holdup BI: 0.31
Normalized holdup BI standard deviation: 0.06
Immobilized partner concentration (10-6M): 13.3
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 4
Measured pKd: 4.57
PUBMED ID: 36115835

Experiment series 3

Protein A

Protein: SCRIB
Fragment: 718-814
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: KCNT2
Fragment: 1126-1135
Site: PBM
Sequence: GQDSREETQL
Construct: biotin-ttds-GQDSREETQL
Measured dissociation constant (10-6M): 26.93
Standard deviation of dissociation constant (10-6M): 1.62
Experiment method: competitive FP
Details of affinity fitting: Competitive binding equation in ProFit
Number of measurements: 3
Measured pKd: 4.57
PUBMED ID: 36115835

Experiment series 4

Protein A

Protein: SCRIB
Fragment: 858-949
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: KCNT2
Fragment: 1126-1135
Site: PBM
Sequence: GQDSREETQL
Construct: biotin-ttds-GQDSREETQL
Average holdup BI: 0.06
Normalized holdup BI: 0.08
Immobilized partner concentration (10-6M): 13.3
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.83
PUBMED ID: 36115835