Interaction Details


Details of experiments between Q14160 and Q5SYB0
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: SCRIB
Fragment: 999-1093
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: FRMPD1
Fragment: 1569-1578
Site: PBM
Sequence: TQKFRASTAL
Construct: Biotin-Ahx-TQKFRASTAL
Average holdup BI: 0.33
Normalized holdup BI: 0.39
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.59
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: SCRIB
Fragment: 999-1093
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: FRMPD1
Fragment: 1569-1578
Site: PBM
Sequence: TQKFRASTAL
Construct: Biotin-Ahx-TQKFRASTAL
Average holdup BI: 0.31
Normalized holdup BI: 0.37
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.54
PUBMED ID: 36115835
Experimental Details

not reported internal standards of holdup layout #6

Experiment series 3

Protein A

Protein: SCRIB
Fragment: 718-814
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: FRMPD1
Fragment: 1569-1578
Site: PBM
Sequence: TQKFRASTAL
Construct: Biotin-Ahx-TQKFRASTAL
Average holdup BI: 0.32
Normalized holdup BI: 0.32
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.46
PUBMED ID: 36115835
Experimental Details

not reported internal standards of holdup layout #6

Experiment series 4

Protein A

Protein: SCRIB
Fragment: 718-814
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: FRMPD1
Fragment: 1569-1578
Site: PBM
Sequence: TQKFRASTAL
Construct: Biotin-Ahx-TQKFRASTAL
Average holdup BI: 0.18
Holdup BI standard deviation: 0.01
Normalized holdup BI: 0.22
Normalized holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 2
Measured pKd: 4.22
PUBMED ID: 36115835

Experiment series 5

Protein A

Protein: SCRIB
Fragment: 858-949
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: FRMPD1
Fragment: 1569-1578
Site: PBM
Sequence: TQKFRASTAL
Construct: Biotin-Ahx-TQKFRASTAL
Average holdup BI: 0.02
Normalized holdup BI: 0.02
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835
Experimental Details

not reported internal standards of holdup layout #6

Experiment series 6

Protein A

Protein: SCRIB
Fragment: 858-949
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: FRMPD1
Fragment: 1569-1578
Site: PBM
Sequence: TQKFRASTAL
Construct: Biotin-Ahx-TQKFRASTAL
Average holdup BI: 0.03
Normalized holdup BI: 0.04
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835