Interaction Details


Details of experiments between Q14160 and Q14155
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: SCRIB
Fragment: 1-1630
Construct: endogenous protein (Jurkat)

Protein B

Protein: ARHGEF7
Fragment: 794-803
Site: PBM
Sequence: NDPAWDETNL
Construct: biotin-ttds-NDPAWDETNL
Experiment method: nHU-WB_Jurkat
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-08-03
Number of measurements: 3
Measured pKd: 5.93
PUBMED ID: 36542723

Experiment series 2

Protein A

Protein: SCRIB
Fragment: 718-814
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: ARHGEF7
Fragment: 794-803
Site: PBM
Sequence: NDPAWDETNL
Construct: biotin-ttds-NDPAWDETNL
Average holdup BI: 0.78
Holdup BI standard deviation: 0
Normalized holdup BI: 0.82
Normalized holdup BI standard deviation: 0
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 2
Measured pKd: 5.5
PUBMED ID: 36115835

Experiment series 3

Protein A

Protein: SCRIB
Fragment: 999-1093
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: ARHGEF7
Fragment: 794-803
Site: PBM
Sequence: NDPAWDETNL
Construct: biotin-ttds-NDPAWDETNL
Average holdup BI: 0.58
Holdup BI standard deviation: 0.04
Normalized holdup BI: 0.65
Normalized holdup BI standard deviation: 0.05
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 2
Measured pKd: 5.08
PUBMED ID: 36115835

Experiment series 4

Protein A

Protein: SCRIB
Fragment: 858-949
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: ARHGEF7
Fragment: 794-803
Site: PBM
Sequence: NDPAWDETNL
Construct: biotin-ttds-NDPAWDETNL
Average holdup BI: 0.35
Holdup BI standard deviation: 0.03
Normalized holdup BI: 0.44
Normalized holdup BI standard deviation: 0.03
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 4
Measured pKd: 4.68
PUBMED ID: 36115835

Experiment series 5

Protein A

Protein: SCRIB
Fragment: 1-1630
Construct: endogenous protein (Jurkat)

Protein B

Protein: ARHGEF7
Fragment: 178-251
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3
Average holdup BI: 0.05
Immobilized partner concentration (10-6M): 10
P value: 0.34
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-06-29
Number of measurements: 3
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680
Experimental Details

-log10(P Two-sided unpaired T-test (6 vs 3))

Experiment series 6

Protein A

Protein: SCRIB
Fragment: 718-814
Site: PDZ_1
Construct: avi(biotin)-his6-MBP-TEVsite-PDZ

Protein B

Protein: ARHGEF7
Fragment: 1-803
Construct: endogenous protein (Jurkat)
Average holdup BI: 0.56
Immobilized partner concentration (10-6M): 10
P value: 1.78
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-08-03
Number of measurements: 3
The affinity was below the detection threshold of the assay.
PUBMED ID: 36542723
Experimental Details

-log10(P Two-sided unpaired T-test (6 vs 3))

Experiment series 7

Protein A

Protein: SCRIB
Fragment: 858-949
Site: PDZ_2
Construct: avi(biotin)-his6-MBP-TEVsite-PDZ

Protein B

Protein: ARHGEF7
Fragment: 1-803
Construct: endogenous protein (Jurkat)
Average holdup BI: 0.29
Immobilized partner concentration (10-6M): 10
P value: 0.84
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-08-03
Number of measurements: 3
The affinity was below the detection threshold of the assay.
PUBMED ID: 36542723
Experimental Details

-log10(P Two-sided unpaired T-test (6 vs 3))