Interaction Details


Details of experiments between Q14160 and P26554
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: SCRIB
Fragment: 999-1093
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV51-E6
Fragment: 142-151
Site: PBM
Sequence: RTRQRNETQV
Construct: biotin-ttds-RTRQRNETQV
Average holdup BI: 0.35
Immobilized partner concentration (10-6M): 7.7
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
Measured pKd: 4.93
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: SCRIB
Fragment: 718-814
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV51-E6
Fragment: 142-151
Site: PBM
Sequence: RTRQRNETQV
Construct: biotin-ttds-RTRQRNETQV
Average holdup BI: 0.26
Immobilized partner concentration (10-6M): 7.7
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
Measured pKd: 4.72
PUBMED ID: 36115835

Experiment series 3

Protein A

Protein: SCRIB
Fragment: 718-814
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV51-E6
Fragment: 142-151
Site: PBM
Sequence: RTRQRNETQV
Construct: biotin-ttds-RTRQRNETQV
Average holdup BI: 0.4
Holdup BI standard deviation: 0.04
Normalized holdup BI: 0.42
Normalized holdup BI standard deviation: 0.05
Immobilized partner concentration (10-6M): 21.5
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 4
Measured pKd: 4.56
PUBMED ID: 36115835

Experiment series 4

Protein A

Protein: SCRIB
Fragment: 718-814
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV51-E6
Fragment: 142-151
Site: PBM
Sequence: RTRQRNETQV
Construct: biotin-ttds-RTRQRNETQV
Measured dissociation constant (10-6M): 30.64
Standard deviation of dissociation constant (10-6M): 0.88
Experiment method: competitive FP
Details of affinity fitting: Competitive binding equation in ProFit
Number of measurements: 3
Measured pKd: 4.51
PUBMED ID: 36115835

Experiment series 5

Protein A

Protein: SCRIB
Fragment: 858-949
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV51-E6
Fragment: 142-151
Site: PBM
Sequence: RTRQRNETQV
Construct: biotin-ttds-RTRQRNETQV
Average holdup BI: 0.27
Normalized holdup BI: 0.33
Immobilized partner concentration (10-6M): 21.5
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.39
PUBMED ID: 36115835

Experiment series 6

Protein A

Protein: SCRIB
Fragment: 1090-1197
Site: PDZ_4
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV51-E6
Fragment: 142-151
Site: PBM
Sequence: RTRQRNETQV
Construct: biotin-ttds-RTRQRNETQV
Average holdup BI: 0.12
Immobilized partner concentration (10-6M): 7.7
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
Measured pKd: 4.27
PUBMED ID: 36115835

Experiment series 7

Protein A

Protein: SCRIB
Fragment: 999-1093
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV51-E6
Fragment: 142-151
Site: PBM
Sequence: RTRQRNETQV
Construct: biotin-ttds-RTRQRNETQV
Average holdup BI: 0.21
Normalized holdup BI: 0.23
Immobilized partner concentration (10-6M): 21.5
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.17
PUBMED ID: 36115835

Experiment series 8

Protein A

Protein: SCRIB
Fragment: 858-949
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV51-E6
Fragment: 142-151
Site: PBM
Sequence: RTRQRNETQV
Construct: biotin-ttds-RTRQRNETQV
Average holdup BI: 0.01
Immobilized partner concentration (10-6M): 7.7
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835