Interaction Details


Details of experiments between Q14160 and P06427
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: SCRIB
Fragment: 718-814
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV33-E6
Fragment: 140-149
Site: PBM
Sequence: SRSRRRETAL
Construct: biotin-ttds-SRSRRRETAL
Average holdup BI: 0.84
Holdup BI standard deviation: 0.02
Normalized holdup BI: 0.88
Normalized holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 20.2
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 8
Measured pKd: 5.66
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: SCRIB
Fragment: 718-814
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV33-E6
Fragment: 140-149
Site: PBM
Sequence: SRSRRRETAL
Construct: biotin-ttds-SRSRRRETAL
Average holdup BI: 0.6
Immobilized partner concentration (10-6M): 7
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
Measured pKd: 5.51
PUBMED ID: 36115835

Experiment series 3

Protein A

Protein: SCRIB
Fragment: 718-814
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV33-E6
Fragment: 140-149
Site: PBM
Sequence: SRSRRRETAL
Construct: biotin-ttds-SRSRRRETAL
Measured dissociation constant (10-6M): 3.6
Standard deviation of dissociation constant (10-6M): 0.37
Experiment method: competitive FP
Details of affinity fitting: Competitive binding equation in ProFit
Number of measurements: 3
Measured pKd: 5.44
PUBMED ID: 36115835

Experiment series 4

Protein A

Protein: SCRIB
Fragment: 999-1093
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV33-E6
Fragment: 140-149
Site: PBM
Sequence: SRSRRRETAL
Construct: biotin-ttds-SRSRRRETAL
Average holdup BI: 0.7
Holdup BI standard deviation: 0.03
Normalized holdup BI: 0.77
Normalized holdup BI standard deviation: 0.03
Immobilized partner concentration (10-6M): 20.2
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 2
Measured pKd: 5.3
PUBMED ID: 36115835

Experiment series 5

Protein A

Protein: SCRIB
Fragment: 999-1093
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV33-E6
Fragment: 140-149
Site: PBM
Sequence: SRSRRRETAL
Construct: biotin-ttds-SRSRRRETAL
Average holdup BI: 0.48
Immobilized partner concentration (10-6M): 7
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
Measured pKd: 5.26
PUBMED ID: 36115835

Experiment series 6

Protein A

Protein: SCRIB
Fragment: 858-949
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV33-E6
Fragment: 140-149
Site: PBM
Sequence: SRSRRRETAL
Construct: biotin-ttds-SRSRRRETAL
Average holdup BI: 0.35
Holdup BI standard deviation: 0
Normalized holdup BI: 0.43
Normalized holdup BI standard deviation: 0
Immobilized partner concentration (10-6M): 20.2
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 2
Measured pKd: 4.61
PUBMED ID: 36115835

Experiment series 7

Protein A

Protein: SCRIB
Fragment: 1090-1197
Site: PDZ_4
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV33-E6
Fragment: 140-149
Site: PBM
Sequence: SRSRRRETAL
Construct: biotin-ttds-SRSRRRETAL
Average holdup BI: 0.1
Immobilized partner concentration (10-6M): 7
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
Measured pKd: 4.24
PUBMED ID: 36115835

Experiment series 8

Protein A

Protein: SCRIB
Fragment: 858-949
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV33-E6
Fragment: 140-149
Site: PBM
Sequence: SRSRRRETAL
Construct: biotin-ttds-SRSRRRETAL
Average holdup BI: 0.06
Immobilized partner concentration (10-6M): 7
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 9

Protein A

Protein: SCRIB
Fragment: 718-814
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV33-E6
Fragment: 140-149
Site: PBM
Modification: (TPO)147
Sequence: SRSRRRETAL
Construct: Biotin-ttds-SRSRRREpTAL
Average holdup BI: -0.01
Normalized holdup BI: -0.01
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 10

Protein A

Protein: SCRIB
Fragment: 858-949
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV33-E6
Fragment: 140-149
Site: PBM
Modification: (TPO)147
Sequence: SRSRRRETAL
Construct: Biotin-ttds-SRSRRREpTAL
Average holdup BI: 0
Normalized holdup BI: 0
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 11

Protein A

Protein: SCRIB
Fragment: 999-1093
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV33-E6
Fragment: 140-149
Site: PBM
Modification: (TPO)147
Sequence: SRSRRRETAL
Construct: Biotin-ttds-SRSRRREpTAL
Average holdup BI: -0.01
Normalized holdup BI: -0.02
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835