Interaction Details


Details of experiments between Q14155 and Q9Y3R0
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: ARHGEF7
Fragment: 794-803
Site: PBM
Sequence: NDPAWDETNL
Construct: biotin-ttds-NDPAWDETNL

Protein B

Protein: GRIP1
Fragment: 145-239
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.13
Holdup BI standard deviation: 0.25
Normalized holdup BI: -0.13
Normalized holdup BI standard deviation: 0.25
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: ARHGEF7
Fragment: 794-803
Site: PBM
Sequence: NDPAWDETNL
Construct: biotin-ttds-NDPAWDETNL

Protein B

Protein: GRIP1
Fragment: 242-344
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.02
Holdup BI standard deviation: 0.03
Normalized holdup BI: 0.02
Normalized holdup BI standard deviation: 0.03
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 3

Protein A

Protein: ARHGEF7
Fragment: 794-803
Site: PBM
Sequence: NDPAWDETNL
Construct: biotin-ttds-NDPAWDETNL

Protein B

Protein: GRIP1
Fragment: 464-567
Site: PDZ_4
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.07
Holdup BI standard deviation: 0.14
Normalized holdup BI: -0.07
Normalized holdup BI standard deviation: 0.14
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 4

Protein A

Protein: ARHGEF7
Fragment: 794-803
Site: PBM
Sequence: NDPAWDETNL
Construct: biotin-ttds-NDPAWDETNL

Protein B

Protein: GRIP1
Fragment: 563-659
Site: PDZ_5
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.08
Holdup BI standard deviation: 0.14
Normalized holdup BI: -0.08
Normalized holdup BI standard deviation: 0.14
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 5

Protein A

Protein: ARHGEF7
Fragment: 794-803
Site: PBM
Sequence: NDPAWDETNL
Construct: biotin-ttds-NDPAWDETNL

Protein B

Protein: GRIP1
Fragment: 996-1086
Site: PDZ_7
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.04
Holdup BI standard deviation: 0.05
Normalized holdup BI: 0.04
Normalized holdup BI standard deviation: 0.05
Immobilized partner concentration (10-6M): 17
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835