Interaction Details


Details of experiments between Q14155 and Q9UQ35
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: ARHGEF7
Fragment: 178-251
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: SRRM2
Fragment: 1-2752
Construct: endogenous protein (Jurkat)
Average holdup BI: 0.39
Immobilized partner concentration (10-6M): 10
P value: 10.22
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-06-29
Number of measurements: 3
Measured pKd: 4.81
PUBMED ID: 38995680
Experimental Details

-log10(P Two-sided unpaired T-test (6 vs 3))

Experiment series 2

Protein A

Protein: ARHGEF7
Fragment: 178-251
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: SRRM2
Fragment: 2029-2043
Site: PxxP_6
Sequence: RSRSRTPLLPRKRSR
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.34
Holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-06-29
Number of measurements: 2
Measured pKd: 4.48
PUBMED ID: 38995680

Experiment series 3

Protein A

Protein: ARHGEF7
Fragment: 178-251
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: SRRM2
Fragment: 2029-2043
Site: PxxP_6
Sequence: RSRSRTPLLPRKRSR
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.34
Holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-06-29
Number of measurements: 2
Measured pKd: 4.48
PUBMED ID: N

Experiment series 4

Protein A

Protein: ARHGEF7
Fragment: 178-251
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: SRRM2
Fragment: 1426-1440
Site: PxxP_2
Sequence: EMKDGLPRTPSRRSR
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.14
Holdup BI standard deviation: 0.04
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-06-29
Number of measurements: 2
Measured pKd: 3.96
PUBMED ID: 38995680

Experiment series 5

Protein A

Protein: ARHGEF7
Fragment: 178-251
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: SRRM2
Fragment: 1426-1440
Site: PxxP_2
Sequence: EMKDGLPRTPSRRSR
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.14
Holdup BI standard deviation: 0.04
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-06-29
Number of measurements: 2
Measured pKd: 3.96
PUBMED ID: N

Experiment series 6

Protein A

Protein: ARHGEF7
Fragment: 178-251
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: SRRM2
Fragment: 1484-1498
Site: PxxP_4
Sequence: PEPKALPQTPRPRSR
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.11
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-06-29
Number of measurements: 2
Measured pKd: 3.85
PUBMED ID: 38995680

Experiment series 7

Protein A

Protein: ARHGEF7
Fragment: 178-251
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: SRRM2
Fragment: 1484-1498
Site: PxxP_4
Sequence: PEPKALPQTPRPRSR
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.11
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-06-29
Number of measurements: 2
Measured pKd: 3.85
PUBMED ID: N

Experiment series 8

Protein A

Protein: ARHGEF7
Fragment: 178-251
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: SRRM2
Fragment: 1405-1419
Site: PxxP_1
Sequence: PVLDAVPRTPSRERS
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.01
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-06-29
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 9

Protein A

Protein: ARHGEF7
Fragment: 178-251
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: SRRM2
Fragment: 1405-1419
Site: PxxP_1
Sequence: PVLDAVPRTPSRERS
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.01
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-06-29
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: N

Experiment series 10

Protein A

Protein: ARHGEF7
Fragment: 178-251
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: SRRM2
Fragment: 1464-1478
Site: PxxP_3
Sequence: PGMKDIPRTPSRGRS
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.04
Holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-06-29
Number of measurements: 4
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 11

Protein A

Protein: ARHGEF7
Fragment: 178-251
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: SRRM2
Fragment: 1464-1478
Site: PxxP_3
Sequence: PGMKDIPRTPSRGRS
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.04
Holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-06-29
Number of measurements: 4
The affinity was below the detection threshold of the assay.
PUBMED ID: N

Experiment series 12

Protein A

Protein: ARHGEF7
Fragment: 178-251
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: SRRM2
Fragment: 1653-1667
Site: PxxP_5
Sequence: SSTESSPEHPPKSRT
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.02
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-06-29
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 13

Protein A

Protein: ARHGEF7
Fragment: 178-251
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: SRRM2
Fragment: 1653-1667
Site: PxxP_5
Sequence: SSTESSPEHPPKSRT
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.02
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-06-29
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: N

Experiment series 14

Protein A

Protein: ARHGEF7
Fragment: 178-251
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: SRRM2
Fragment: 2405-2419
Site: PxxP_7
Sequence: ARSRTPPSAPSQSRM
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.02
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-06-29
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 15

Protein A

Protein: ARHGEF7
Fragment: 178-251
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: SRRM2
Fragment: 2405-2419
Site: PxxP_7
Sequence: ARSRTPPSAPSQSRM
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.02
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-06-29
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: N

Experiment series 16

Protein A

Protein: ARHGEF7
Fragment: 178-251
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: SRRM2
Fragment: 2656-2670
Site: PxxP_8
Sequence: PQALPKPASPKKPPP
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.03
Holdup BI standard deviation: 0.04
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-06-29
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 17

Protein A

Protein: ARHGEF7
Fragment: 178-251
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: SRRM2
Fragment: 2656-2670
Site: PxxP_8
Sequence: PQALPKPASPKKPPP
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.03
Holdup BI standard deviation: 0.04
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-06-29
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: N

Experiment series 18

Protein A

Protein: ARHGEF7
Fragment: 178-251
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: SRRM2
Fragment: 400-414
Site: PxxP_9
Sequence: TRDRSPPKSPEKLPQ
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.01
Holdup BI standard deviation: 0.04
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-06-29
Number of measurements: 4
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 19

Protein A

Protein: ARHGEF7
Fragment: 178-251
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: SRRM2
Fragment: 400-414
Site: PxxP_9
Sequence: TRDRSPPKSPEKLPQ
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.01
Holdup BI standard deviation: 0.04
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-06-29
Number of measurements: 4
The affinity was below the detection threshold of the assay.
PUBMED ID: N

Experiment series 20

Protein A

Protein: ARHGEF7
Fragment: 178-251
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: SRRM2
Fragment: 975-989
Site: PxxP_10
Sequence: PDTKVKPETPPRQSH
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.03
Holdup BI standard deviation: 0.05
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-06-29
Number of measurements: 4
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 21

Protein A

Protein: ARHGEF7
Fragment: 178-251
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: SRRM2
Fragment: 975-989
Site: PxxP_10
Sequence: PDTKVKPETPPRQSH
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.03
Holdup BI standard deviation: 0.05
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-06-29
Number of measurements: 4
The affinity was below the detection threshold of the assay.
PUBMED ID: N