Interaction Details


Details of experiments between Q14155 and Q96P48
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: ARHGEF7
Fragment: 178-251
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: ARAP1
Fragment: 1-1450
Construct: endogenous protein (Jurkat)
Average holdup BI: 0.74
Immobilized partner concentration (10-6M): 10
P value: 8
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-06-29
Number of measurements: 3
Measured pKd: 5.45
PUBMED ID: 38995680
Experimental Details

-log10(P Two-sided unpaired T-test (6 vs 3))

Experiment series 2

Protein A

Protein: ARHGEF7
Fragment: 178-251
Site: SH3
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: ARAP1
Fragment: 109-123
Site: PxxP_1
Sequence: EGLPAAPPIPPRRSC
Construct: Bio Ado Ado-EGLPAAPPIPPRRSC-CONH2
Average holdup BI: 0.76
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
Measured pKd: 5.34
PUBMED ID: 38995680

Experiment series 3

Protein A

Protein: ARHGEF7
Fragment: 178-251
Site: SH3
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: ARAP1
Fragment: 110-124
Site: PxxP_2
Sequence: GLPAAPPIPPRRSCL
Construct: Bio Ado Ado-GLPAAPPIPPRRSCL-CONH2
Average holdup BI: 0.63
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
Measured pKd: 5.05
PUBMED ID: 38995680

Experiment series 4

Protein A

Protein: ARHGEF7
Fragment: 178-251
Site: SH3
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: ARAP1
Fragment: 210-224
Site: PxxP_6
Sequence: PCPPEIPPKPVRLFP
Construct: Bio Ado Ado-PCPPEIPPKPVRLFP-CONH2
Average holdup BI: 0.58
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
Measured pKd: 4.95
PUBMED ID: 38995680

Experiment series 5

Protein A

Protein: ARHGEF7
Fragment: 178-251
Site: SH3
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: ARAP1
Fragment: 150-164
Site: PxxP_4
Sequence: LAELSVPPVPPRTGP
Construct: Bio Ado Ado-LAELSVPPVPPRTGP-CONH2
Average holdup BI: 0.36
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
Measured pKd: 4.54
PUBMED ID: 38995680

Experiment series 6

Protein A

Protein: ARHGEF7
Fragment: 178-251
Site: SH3
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: ARAP1
Fragment: 136-150
Site: PxxP_3
Sequence: APDPVLPPLPAKRHL
Construct: Bio Ado Ado-APDPVLPPLPAKRHL-CONH2
Average holdup BI: 0.28
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
Measured pKd: 4.35
PUBMED ID: 38995680

Experiment series 7

Protein A

Protein: ARHGEF7
Fragment: 178-251
Site: SH3
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: ARAP1
Fragment: 207-221
Site: PxxP_5
Sequence: SPPPCPPEIPPKPVR
Construct: Bio Ado Ado-SPPPCPPEIPPKPVR-CONH2
Average holdup BI: 0.14
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
Measured pKd: 3.97
PUBMED ID: 38995680

Experiment series 8

Protein A

Protein: ARHGEF7
Fragment: 178-251
Site: SH3
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: ARAP1
Fragment: 233-247
Site: PxxP_7
Sequence: EVPEEGPGAPARVMT
Construct: Bio Ado Ado-EVPEEGPGAPARVMT-CONH2
Average holdup BI: 0.03
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680