Interaction Details


Details of experiments between Q14155 and Q96L92
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: ARHGEF7
Fragment: 794-803
Site: PBM
Sequence: NDPAWDETNL
Construct: biotin-ttds-NDPAWDETNL

Protein B

Protein: SNX27
Fragment: 1-541
Construct: endogenous protein (Jurkat)
Experiment method: nHU-WB_Jurkat
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-08-03
Number of measurements: 3
Measured pKd: 5.82
PUBMED ID: 36542723

Experiment series 2

Protein A

Protein: ARHGEF7
Fragment: 794-803
Site: PBM
Sequence: NDPAWDETNL
Construct: biotin-ttds-NDPAWDETNL

Protein B

Protein: SNX27
Fragment: 40-141
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.87
Holdup BI standard deviation: 0
Normalized holdup BI: 0.87
Normalized holdup BI standard deviation: 0
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 2
Measured pKd: 5.66
PUBMED ID: 36115835

Experiment series 3

Protein A

Protein: ARHGEF7
Fragment: 1-803
Construct: endogenous protein (Jurkat)

Protein B

Protein: SNX27
Fragment: 40-141
Site: PDZ
Construct: avi(biotin)-his6-MBP-TEVsite-PDZ
Average holdup BI: 0.8
Immobilized partner concentration (10-6M): 10
P value: 2.97
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-06-27
Number of measurements: 6
Measured pKd: 5.61
PUBMED ID: 36542723
Experimental Details

-log10(P Two-sided unpaired T-test (6 vs 6))

Experiment series 4

Protein A

Protein: ARHGEF7
Fragment: 1-803
Construct: endogenous protein (SH-SY5Y)

Protein B

Protein: SNX27
Fragment: 40-141
Site: PDZ
Construct: avi(biotin)-his6-MBP-TEVsite-PDZ
Average holdup BI: 0.74
Immobilized partner concentration (10-6M): 10
P value: 2.97
Experiment method: nHU_SH-SY5Y
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-06-27
Number of measurements: 6
Measured pKd: 5.46
PUBMED ID: 36542723
Experimental Details

-log10(P Two-sided unpaired T-test (6 vs 6))

Experiment series 5

Protein A

Protein: ARHGEF7
Fragment: 1-803
Construct: endogenous protein (Jurkat)

Protein B

Protein: SNX27
Fragment: 40-141
Site: PDZ
Construct: avi(biotin)-his6-MBP-TEVsite-PDZ
Average holdup BI: 0.69
Immobilized partner concentration (10-6M): 10
P value: 2.4
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-08-03
Number of measurements: 3
Measured pKd: 5.36
PUBMED ID: 36542723
Experimental Details

-log10(P Two-sided unpaired T-test (6 vs 3))

Experiment series 6

Protein A

Protein: ARHGEF7
Fragment: 178-251
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: SNX27
Fragment: 1-541
Construct: endogenous protein (Jurkat)
Average holdup BI: -0.09
Immobilized partner concentration (10-6M): 10
P value: 1.01
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-06-29
Number of measurements: 3
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680
Experimental Details

-log10(P Two-sided unpaired T-test (6 vs 3))