Interaction Details


Details of experiments between Q14155 and Q8TEW0
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: ARHGEF7
Fragment: 794-803
Site: PBM
Sequence: NDPAWDETNL
Construct: biotin-ttds-NDPAWDETNL

Protein B

Protein: PARD3
Fragment: 582-685
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.19
Holdup BI standard deviation: 0.06
Normalized holdup BI: 0.19
Normalized holdup BI standard deviation: 0.06
Immobilized partner concentration (10-6M): 17
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 2
Measured pKd: 4.16
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: ARHGEF7
Fragment: 178-251
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: PARD3
Fragment: 1-1356
Construct: endogenous protein (Jurkat)
Average holdup BI: -0.01
Immobilized partner concentration (10-6M): 10
P value: 0.07
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-06-29
Number of measurements: 3
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680
Experimental Details

-log10(P Two-sided unpaired T-test (6 vs 3))