Interaction Details


Details of experiments between Q14155 and Q5SQI0
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: ARHGEF7
Fragment: 178-251
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: ATAT1
Fragment: 1-421
Construct: endogenous protein (Jurkat)
Average holdup BI: 0.86
Immobilized partner concentration (10-6M): 10
P value: 7.65
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-06-29
Number of measurements: 3
Measured pKd: 5.8
PUBMED ID: 38995680
Experimental Details

-log10(P Two-sided unpaired T-test (6 vs 3))

Experiment series 2

Protein A

Protein: ARHGEF7
Fragment: 178-251
Site: SH3
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: ATAT1
Fragment: 210-224
Site: PxxP_1
Sequence: AAVDPTPAAPARKLP
Construct: Bio Ado Ado-AAVDPTPAAPARKLP-CONH2
Average holdup BI: 0.09
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
Measured pKd: 3.75
PUBMED ID: 38995680

Experiment series 3

Protein A

Protein: ARHGEF7
Fragment: 178-251
Site: SH3
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: ATAT1
Fragment: 293-307
Site: PxxP_2
Sequence: RSLRLCPPHPTARLL
Construct: Bio Ado Ado-RSLRLCPPHPTARLL-CONH2
Average holdup BI: 0.06
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
Measured pKd: 3.55
PUBMED ID: 38995680