Interaction Details


Details of experiments between Q14155 and Q5JSH3
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: ARHGEF7
Fragment: 178-251
Site: SH3
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: WDR44
Fragment: 247-261
Site: PxxP_4
Sequence: PPPPSRPAPPPRKRK
Construct: Bio Ado Ado-PPPPSRPAPPPRKRK-CONH2
Average holdup BI: 0.38
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
Measured pKd: 4.58
PUBMED ID: 38995680

Experiment series 2

Protein A

Protein: ARHGEF7
Fragment: 178-251
Site: SH3
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: WDR44
Fragment: 205-219
Site: PxxP_1
Sequence: AVEEVAPAKPPRHLT
Construct: Bio Ado Ado-AVEEVAPAKPPRHLT-CONH2
Average holdup BI: 0.18
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
Measured pKd: 4.12
PUBMED ID: 38995680

Experiment series 3

Protein A

Protein: ARHGEF7
Fragment: 178-251
Site: SH3
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: WDR44
Fragment: 241-255
Site: PxxP_3
Sequence: NFPPPRPPPPSRPAP
Construct: Bio Ado Ado-NFPPPRPPPPSRPAP-CONH2
Average holdup BI: 0.09
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
Measured pKd: 3.76
PUBMED ID: 38995680

Experiment series 4

Protein A

Protein: ARHGEF7
Fragment: 178-251
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: WDR44
Fragment: 1-913
Construct: endogenous protein (Jurkat)
Average holdup BI: 0.11
Immobilized partner concentration (10-6M): 10
P value: 1.33
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-06-29
Number of measurements: 3
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680
Experimental Details

-log10(P Two-sided unpaired T-test (6 vs 3))

Experiment series 5

Protein A

Protein: ARHGEF7
Fragment: 178-251
Site: SH3
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: WDR44
Fragment: 227-241
Site: PxxP_2
Sequence: STKKPVPARPPPPTN
Construct: Bio Ado Ado-STKKPVPARPPPPTN-CONH2
Average holdup BI: 0.02
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680