Interaction Details


Details of experiments between Q14155 and Q13283
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: ARHGEF7
Fragment: 178-251
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: G3BP1
Fragment: 1-466
Construct: endogenous protein (Jurkat)
Average holdup BI: 0.47
Immobilized partner concentration (10-6M): 10
P value: 10.17
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-06-29
Number of measurements: 3
Measured pKd: 4.95
PUBMED ID: 38995680
Experimental Details

-log10(P Two-sided unpaired T-test (6 vs 3))

Experiment series 2

Protein A

Protein: ARHGEF7
Fragment: 178-251
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: G3BP1
Fragment: 288-302
Site: PxxP_1
Sequence: PESQIPPQRPQRDQR
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.07
Holdup BI standard deviation: 0.05
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-06-29
Number of measurements: 2
Measured pKd: 3.62
PUBMED ID: 38995680

Experiment series 3

Protein A

Protein: ARHGEF7
Fragment: 178-251
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: G3BP1
Fragment: 288-302
Site: PxxP_1
Sequence: PESQIPPQRPQRDQR
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.07
Holdup BI standard deviation: 0.05
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-06-29
Number of measurements: 2
Measured pKd: 3.62
PUBMED ID: N