Interaction Details


Details of experiments between Q14155 and P61978
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: ARHGEF7
Fragment: 178-251
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: HNRNPK
Fragment: 1-463
Construct: endogenous protein (Jurkat)
Average holdup BI: 0.31
Immobilized partner concentration (10-6M): 10
P value: 7.94
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-06-29
Number of measurements: 3
Measured pKd: 4.65
PUBMED ID: 38995680
Experimental Details

-log10(P Two-sided unpaired T-test (6 vs 3))

Experiment series 2

Protein A

Protein: ARHGEF7
Fragment: 178-251
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: HNRNPK
Fragment: 302-316
Site: PxxP_5
Sequence: SRARNLPLPPPPPPR
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.36
Holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-06-29
Number of measurements: 2
Measured pKd: 4.54
PUBMED ID: 38995680

Experiment series 3

Protein A

Protein: ARHGEF7
Fragment: 178-251
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: HNRNPK
Fragment: 302-316
Site: PxxP_5
Sequence: SRARNLPLPPPPPPR
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.36
Holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-06-29
Number of measurements: 2
Measured pKd: 4.54
PUBMED ID: N

Experiment series 4

Protein A

Protein: ARHGEF7
Fragment: 178-251
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: HNRNPK
Fragment: 285-299
Site: PxxP_4
Sequence: PRRGPPPPPPGRGGR
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.34
Holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-06-29
Number of measurements: 2
Measured pKd: 4.48
PUBMED ID: 38995680

Experiment series 5

Protein A

Protein: ARHGEF7
Fragment: 178-251
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: HNRNPK
Fragment: 285-299
Site: PxxP_4
Sequence: PRRGPPPPPPGRGGR
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.34
Holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-06-29
Number of measurements: 2
Measured pKd: 4.48
PUBMED ID: N

Experiment series 6

Protein A

Protein: ARHGEF7
Fragment: 178-251
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: HNRNPK
Fragment: 284-298
Site: PxxP_3
Sequence: SPRRGPPPPPPGRGG
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.33
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-06-29
Number of measurements: 2
Measured pKd: 4.46
PUBMED ID: 38995680

Experiment series 7

Protein A

Protein: ARHGEF7
Fragment: 178-251
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: HNRNPK
Fragment: 284-298
Site: PxxP_3
Sequence: SPRRGPPPPPPGRGG
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.33
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-06-29
Number of measurements: 2
Measured pKd: 4.46
PUBMED ID: N

Experiment series 8

Protein A

Protein: ARHGEF7
Fragment: 178-251
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: HNRNPK
Fragment: 283-297
Site: PxxP_2
Sequence: MSPRRGPPPPPPGRG
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.26
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-06-29
Number of measurements: 2
Measured pKd: 4.31
PUBMED ID: 38995680

Experiment series 9

Protein A

Protein: ARHGEF7
Fragment: 178-251
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: HNRNPK
Fragment: 283-297
Site: PxxP_2
Sequence: MSPRRGPPPPPPGRG
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.26
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-06-29
Number of measurements: 2
Measured pKd: 4.31
PUBMED ID: N

Experiment series 10

Protein A

Protein: ARHGEF7
Fragment: 178-251
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: HNRNPK
Fragment: 305-319
Site: PxxP_6
Sequence: RNLPLPPPPPPRGGD
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.2
Holdup BI standard deviation: 0.05
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-06-29
Number of measurements: 2
Measured pKd: 4.15
PUBMED ID: 38995680

Experiment series 11

Protein A

Protein: ARHGEF7
Fragment: 178-251
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: HNRNPK
Fragment: 305-319
Site: PxxP_6
Sequence: RNLPLPPPPPPRGGD
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.2
Holdup BI standard deviation: 0.05
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-06-29
Number of measurements: 2
Measured pKd: 4.15
PUBMED ID: N

Experiment series 12

Protein A

Protein: ARHGEF7
Fragment: 178-251
Site: SH3
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: HNRNPK
Fragment: 285-299
Site: PxxP_4
Sequence: PRRGPPPPPPGRGGR
Construct: Bio E E Ado Ado-PRRGPPPPPPGRGGR-CONH2
Average holdup BI: 0.1
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
Measured pKd: 3.79
PUBMED ID: 38995680

Experiment series 13

Protein A

Protein: ARHGEF7
Fragment: 178-251
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: HNRNPK
Fragment: 266-280
Site: PxxP_1
Sequence: PGRGGRPMPPSRRDY
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.05
Holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-06-29
Number of measurements: 2
Measured pKd: 3.5
PUBMED ID: 38995680

Experiment series 14

Protein A

Protein: ARHGEF7
Fragment: 178-251
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: HNRNPK
Fragment: 266-280
Site: PxxP_1
Sequence: PGRGGRPMPPSRRDY
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.05
Holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-06-29
Number of measurements: 2
Measured pKd: 3.5
PUBMED ID: N