Interaction Details


Details of experiments between Q14155 and P56282
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: ARHGEF7
Fragment: 1-803
Construct: endogenous proteins (Jurkat)

Protein B

Protein: POLE2
Fragment: 1-527
Construct: avi-his6-MBP-fusion protein
Average holdup BI: 0.01
Immobilized partner concentration (10-6M): 26
P value: 0.14
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic equation
Date of measurement: 2024-02-02
Number of measurements: 6
The affinity was below the detection threshold of the assay.
Experimental Details

Batch = Helsinki_batch6; Ncontrols = 12; Nsamples = 6; Npreys = 6515; threshold curvature: 2.18

Experiment series 2

Protein A

Protein: ARHGEF7
Fragment: 178-251
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: POLE2
Fragment: 1-527
Construct: endogenous protein (Jurkat)
Average holdup BI: 0.03
Immobilized partner concentration (10-6M): 10
P value: 0.09
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-06-29
Number of measurements: 3
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680
Experimental Details

-log10(P Two-sided unpaired T-test (6 vs 3))