Interaction Details


Details of experiments between Q14155 and P38159
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: ARHGEF7
Fragment: 178-251
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: RBMX
Fragment: 1-391
Construct: endogenous protein (Jurkat)
Average holdup BI: 0.33
Immobilized partner concentration (10-6M): 10
P value: 5.27
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-06-29
Number of measurements: 3
Measured pKd: 4.69
PUBMED ID: 38995680
Experimental Details

-log10(P Two-sided unpaired T-test (6 vs 3))

Experiment series 2

Protein A

Protein: ARHGEF7
Fragment: 178-251
Site: SH3
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: RBMX
Fragment: 90-104
Site: PxxP_2
Sequence: ESGRRGPPPPPRSRG
Construct: Bio Ado Ado-ESGRRGPPPPPRSRG-CONH2
Average holdup BI: 0.3
Holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 2
Measured pKd: 4.4
PUBMED ID: 38995680

Experiment series 3

Protein A

Protein: ARHGEF7
Fragment: 178-251
Site: SH3
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: RBMX
Fragment: 91-105
Site: PxxP_3
Sequence: SGRRGPPPPPRSRGP
Construct: Bio Ado Ado-SGRRGPPPPPRSRGP-CONH2
Average holdup BI: 0.26
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
Measured pKd: 4.32
PUBMED ID: 38995680

Experiment series 4

Protein A

Protein: ARHGEF7
Fragment: 178-251
Site: SH3
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: RBMX
Fragment: 161-175
Site: PxxP_1
Sequence: PPKRSAPSGPVRSSS
Construct: Bio Ado Ado-PPKRSAPSGPVRSSS-CONH2
Average holdup BI: -0.02
Holdup BI standard deviation: 0.03
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680