Interaction Details


Details of experiments between Q14155 and P30260
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: ARHGEF7
Fragment: 178-251
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: CDC27
Fragment: 396-410
Site: PxxP_1
Sequence: LKMKFPPKIPNRKTK
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.22
Holdup BI standard deviation: 0.04
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-06-29
Number of measurements: 4
Measured pKd: 4.23
PUBMED ID: 38995680

Experiment series 2

Protein A

Protein: ARHGEF7
Fragment: 178-251
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: CDC27
Fragment: 396-410
Site: PxxP_1
Sequence: LKMKFPPKIPNRKTK
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.22
Holdup BI standard deviation: 0.04
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-06-29
Number of measurements: 4
Measured pKd: 4.23
PUBMED ID: N

Experiment series 3

Protein A

Protein: ARHGEF7
Fragment: 178-251
Site: SH3
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: CDC27
Fragment: 396-410
Site: PxxP_1
Sequence: LKMKFPPKIPNRKTK
Construct: Bio E E Ado Ado-LKMKFPPKIPNRKTK-CONH2
Average holdup BI: 0.16
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
Measured pKd: 4.04
PUBMED ID: 38995680

Experiment series 4

Protein A

Protein: ARHGEF7
Fragment: 178-251
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: CDC27
Fragment: 1-824
Construct: endogenous protein (Jurkat)
Average holdup BI: 0.19
Immobilized partner concentration (10-6M): 10
P value: 5.08
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-06-29
Number of measurements: 3
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680
Experimental Details

-log10(P Two-sided unpaired T-test (6 vs 3))