Interaction Details


Details of experiments between Q13884 and Q15418
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: SNTB1
Fragment: 107-196
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: RPS6KA1
Fragment: 729-735
Site: PBM
Sequence: RVRKLPSTTL
Construct: Fluorescein-KLPSTTL
Measured dissociation constant (10-6M): 18
Standard deviation of dissociation constant (10-6M): 2
Experiment method: FP_direct
Number of measurements: 3
Measured pKd: 4.74
PUBMED ID: 30726710

Experiment series 2

Protein A

Protein: SNTB1
Fragment: 107-196
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: RPS6KA1
Fragment: 683-735
Site: PBM
Sequence: RVRKLPSTTL
Construct: ...KLPSTTL
Measured dissociation constant (10-6M): 37
Standard deviation of dissociation constant (10-6M): 6
Experiment method: FP_competitive
Number of measurements: 3
Measured pKd: 4.43
PUBMED ID: 30726710

Experiment series 3

Protein A

Protein: SNTB1
Fragment: 107-196
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: RPS6KA1
Fragment: 725-735
Site: PBM
Sequence: RVRKLPSTTL
Construct: biotin-ttds-RRVRKLPSTTL
Measured dissociation constant (10-6M): 55.5
Standard deviation of dissociation constant (10-6M): 20.02
Experiment method: competitive FP
Details of affinity fitting: Competitive binding equation in ProFit
Number of measurements: 3
Measured pKd: 4.26
PUBMED ID: 36115835

Experiment series 4

Protein A

Protein: SNTB1
Fragment: 107-196
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: RPS6KA1
Fragment: 726-735
Site: PBM
Sequence: RVRKLPSTTL
Construct: Biotin-ado-ado-RVRKLPSTTL
Average holdup BI: 0.21
Normalized holdup BI: 0.23
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.25
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6

Experiment series 5

Protein A

Protein: SNTB1
Fragment: 107-196
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: RPS6KA1
Fragment: 725-735
Site: PBM
Sequence: RVRKLPSTTL
Construct: biotin-ttds-RRVRKLPSTTL
Average holdup BI: 0.16
Holdup BI standard deviation: 0.04
Normalized holdup BI: 0.19
Normalized holdup BI standard deviation: 0.05
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 2
Measured pKd: 4.14
PUBMED ID: 36115835

Experiment series 6

Protein A

Protein: SNTB1
Fragment: 107-196
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: RPS6KA1
Fragment: 725-735
Site: PBM
Sequence: RVRKLPSTTL
Construct: biotin-ttds-RRVRKLPSTTL
Average holdup BI: 0.2
Holdup BI standard deviation: 0.06
Immobilized partner concentration (10-6M): 31
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 4
Measured pKd: 3.92
PUBMED ID: 36115835

Experiment series 7

Protein A

Protein: SNTB1
Fragment: 107-196
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: RPS6KA1
Fragment: 725-735
Site: PBM
Sequence: RVRKLPSTTL
Construct: Biotin-ttds-RRVRKLPSTTL
Measured dissociation constant (10-6M): 0
Experiment method: SPR
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 30726710

Experiment series 8

Protein A

Protein: SNTB1
Fragment: 107-196
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: RPS6KA1
Fragment: 729-735
Site: PBM
Modification: (SEP)732
Sequence: RVRKLPSTTL
Construct: Fluorescein-KLP(pS)TTL
Measured dissociation constant (10-6M): 1.5
Standard deviation of dissociation constant (10-6M): 0.1
Experiment method: FP_direct
Number of measurements: 3
Measured pKd: 5.82
PUBMED ID: 30726710

Experiment series 9

Protein A

Protein: SNTB1
Fragment: 107-196
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: RPS6KA1
Fragment: 683-735
Site: PBM
Modification: (SEP)732
Sequence: RVRKLPSTTL
Construct: ...KLP(pS)TTL
Measured dissociation constant (10-6M): 4.5
Standard deviation of dissociation constant (10-6M): 0.3
Experiment method: FP_competitive
Number of measurements: 3
Measured pKd: 5.35
PUBMED ID: 30726710

Experiment series 10

Protein A

Protein: SNTB1
Fragment: 107-196
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: RPS6KA1
Fragment: 725-735
Site: PBM
Modification: (SEP)732
Sequence: RVRKLPSTTL
Construct: biotin-ttds-RRVRKLPpSTTL
Measured dissociation constant (10-6M): 19.19
Standard deviation of dissociation constant (10-6M): 1.39
Experiment method: competitive FP
Details of affinity fitting: Competitive binding equation in ProFit
Number of measurements: 3
Measured pKd: 4.72
PUBMED ID: 36115835

Experiment series 11

Protein A

Protein: SNTB1
Fragment: 107-196
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: RPS6KA1
Fragment: 725-735
Site: PBM
Modification: (SEP)732
Sequence: RVRKLPSTTL
Construct: biotin-ttds-RRVRKLPpSTTL
Average holdup BI: 0.3
Normalized holdup BI: 0.33
Immobilized partner concentration (10-6M): 15
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.56
Experimental Details

not reported internal standards of holdup layout #5

Experiment series 12

Protein A

Protein: SNTB1
Fragment: 107-196
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: RPS6KA1
Fragment: 725-735
Site: PBM
Modification: (SEP)732
Sequence: RVRKLPSTTL
Construct: biotin-ttds-RRVRKLPpSTTL
Average holdup BI: 0.21
Holdup BI standard deviation: 0.09
Normalized holdup BI: 0.26
Normalized holdup BI standard deviation: 0.11
Immobilized partner concentration (10-6M): 15
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 2
Measured pKd: 4.39
PUBMED ID: 36115835

Experiment series 13

Protein A

Protein: SNTB1
Fragment: 107-196
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: RPS6KA1
Fragment: 725-735
Site: PBM
Modification: (SEP)732
Sequence: RVRKLPSTTL
Construct: biotin-ttds-RRVRKLPpSTTL
Average holdup BI: 0.48
Holdup BI standard deviation: 0.07
Immobilized partner concentration (10-6M): 40
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 3
Measured pKd: 4.38
PUBMED ID: 36115835

Experiment series 14

Protein A

Protein: SNTB1
Fragment: 107-196
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: RPS6KA1
Fragment: 725-735
Site: PBM
Modification: (SEP)732
Sequence: RVRKLPSTTL
Construct: Biotin-ttds-RRVRKLP(pS)TTL
Measured dissociation constant (10-6M): 48
Standard deviation of dissociation constant (10-6M): 5
Experiment method: SPR
Number of measurements: 1
Measured pKd: 4.32
PUBMED ID: 30726710

Experiment series 15

Protein A

Protein: SNTB1
Fragment: 107-196
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: RPS6KA1
Fragment: 725-735
Site: PBM
Modification: (TPO)733
Sequence: RVRKLPSTTL
Construct: biotin-ttds-RRVRKLPSpTTL
Average holdup BI: 0.03
Holdup BI standard deviation: 0.08
Normalized holdup BI: 0.04
Normalized holdup BI standard deviation: 0.1
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 16

Protein A

Protein: SNTB1
Fragment: 107-196
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: RPS6KA1
Fragment: 725-735
Site: PBM
Modification: (TPO)733
Sequence: RVRKLPSTTL
Construct: biotin-ttds-RRVRKLPSpTTL
Measured dissociation constant (10-6M): 0
Experiment method: competitive FP
Details of affinity fitting: Competitive binding equation in ProFit
Number of measurements: 3
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 17

Protein A

Protein: SNTB1
Fragment: 107-196
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: RPS6KA1
Fragment: 725-735
Site: PBM
Modification: (TPO)734
Sequence: RVRKLPSTTL
Construct: Biotin-ttds-RRVRKLPSTpTL
Average holdup BI: 0.15
Normalized holdup BI: 0.17
Immobilized partner concentration (10-6M): 25
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.91
PUBMED ID: 36115835

Experiment series 18

Protein A

Protein: SNTB1
Fragment: 107-196
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: RPS6KA1
Fragment: 725-735
Site: PBM
Modification: (TPO)734
Sequence: RVRKLPSTTL
Construct: Biotin-ttds-RRVRKLPSTpTL
Measured dissociation constant (10-6M): 0
Experiment method: competitive FP
Details of affinity fitting: Competitive binding equation in ProFit
Number of measurements: 3
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835