Interaction Details


Details of experiments between Q13884 and P03126
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: SNTB1
Fragment: 107-196
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV16-E6
Fragment: 149-158
Site: PBM
Sequence: SSRTRRETQL
Construct: biotin-ttds-SSRTRRETQL
Measured dissociation constant (10-6M): 9.75
Standard deviation of dissociation constant (10-6M): 0.9
Experiment method: competitive FP
Details of affinity fitting: Competitive binding equation in ProFit
Number of measurements: 3
Measured pKd: 5.01
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: SNTB1
Fragment: 107-196
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV16-E6
Fragment: 149-158
Site: PBM
Sequence: SSRTRRETQL
Construct: biotin-ttds-SSRTRRETQL
Average holdup BI: 0.47
Holdup BI standard deviation: 0.05
Normalized holdup BI: 0.57
Normalized holdup BI standard deviation: 0.06
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 2
Measured pKd: 4.92
PUBMED ID: 36115835

Experiment series 3

Protein A

Protein: SNTB1
Fragment: 107-196
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV16-E6
Fragment: 149-158
Site: PBM
Sequence: SSRTRRETQL
Construct: biotin-ttds-SSRTRRETQL
Average holdup BI: 0.5
Holdup BI standard deviation: 0.19
Immobilized partner concentration (10-6M): 15
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 6
Measured pKd: 4.88
PUBMED ID: 36115835

Experiment series 4

Protein A

Protein: SNTB1
Fragment: 107-196
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV16-E6
Fragment: 149-158
Site: PBM
Sequence: SSRTRRETQL
Construct: Biotin-ado-ado-SSRTRRETQL
Average holdup BI: 0.48
Normalized holdup BI: 0.54
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.87
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6

Experiment series 5

Protein A

Protein: SNTB1
Fragment: 107-196
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV16-E6
Fragment: 149-158
Site: PBM
Sequence: SSRTRRETQL
Construct: biotin-ttds-SSRTRRETQL
Average holdup BI: 0.4
Normalized holdup BI: 0.44
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.68
Experimental Details

not reported internal standards of holdup layout #5

Experiment series 6

Protein A

Protein: SNTB1
Fragment: 107-196
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV16-E6
Fragment: 149-158
Site: PBM
Sequence: SSRTRRETQL
Construct: Biotin-ttds-SSRTRRETQL
Measured dissociation constant (10-6M): 27
Standard deviation of dissociation constant (10-6M): 4
Experiment method: SPR
Number of measurements: 1
Measured pKd: 4.57
PUBMED ID: 30726710

Experiment series 7

Protein A

Protein: SNTB1
Fragment: 107-196
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV16-E6
Fragment: 149-158
Site: PBM
Modification: (TPO)156
Sequence: SSRTRRETQL
Construct: Biotin-ttds-SSRTRREpTQL
Average holdup BI: -0.06
Immobilized partner concentration (10-6M): 18
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 8

Protein A

Protein: SNTB1
Fragment: 107-196
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV16-E6
Fragment: 149-158
Site: PBM
Modification: (TPO)156
Sequence: SSRTRRETQL
Construct: Biotin-ttds-SSRTRREpTQL
Average holdup BI: -0.06
Normalized holdup BI: -0.06
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 9

Protein A

Protein: SNTB1
Fragment: 107-196
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV16-E6
Fragment: 149-158
Site: PBM
Modification: (TPO)156
Sequence: SSRTRRETQL
Construct: Biotin-ttds-SSRTRREpTQL
Measured dissociation constant (10-6M): 0
Experiment method: competitive FP
Details of affinity fitting: Competitive binding equation in ProFit
Number of measurements: 3
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835