Interaction Details

Details of experiments between Q13884 and O00592
Note that the results of all experiments are listed, regardless of the modification states of the fragments.

Experiment series 1

Protein A

Protein: SNTB1
Fragment: 107-196
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: PODXL
Fragment: 549-558
Site: PBM
Sequence: DLDEEEDTHL
Construct: biotin-ttds-DLDEEEDTHL

Average holdup BI: 0.03

Holdup BI standard deviation: 0.08

Normalized holdup BI: 0.04

Normalized holdup BI standard deviation: 0.1

Immobilized partner concentration (10^-6M): 18

Experiment method: DAPF_holdup

Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout

Number of measurements: 2

The affinity was below the detection threshold of the assay.

PUBMED ID: 36115835