Interaction Details


Details of experiments between Q13613 and Q92796
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: MTMR1
Fragment: 656-665
Site: PBM
Sequence: HSATSVHTSV
Construct: Biotin-Ahx-HSATSVHTSV

Protein B

Protein: DLG3
Fragment: 221-314
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.38
Normalized holdup BI: 0.46
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.72
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: MTMR1
Fragment: 656-665
Site: PBM
Sequence: HSATSVHTSV
Construct: Biotin-Ahx-HSATSVHTSV

Protein B

Protein: DLG3
Fragment: 124-219
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.12
Normalized holdup BI: 0.12
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.9
PUBMED ID: 36115835

Experiment series 3

Protein A

Protein: MTMR1
Fragment: 656-665
Site: PBM
Sequence: HSATSVHTSV
Construct: Biotin-Ahx-HSATSVHTSV

Protein B

Protein: DLG3
Fragment: 381-476
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.06
Normalized holdup BI: 0.08
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.68
PUBMED ID: 36115835