Interaction Details


Details of experiments between Q13574 and Q9NY99
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: DGKZ
Fragment: 919-928
Site: PBM
Sequence: IQREDQETAV
Construct: biotin-ttds-IQREDQETAV

Protein B

Protein: SNTG2
Fragment: 70-159
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.43
Holdup BI standard deviation: 0.03
Normalized holdup BI: 0.51
Normalized holdup BI standard deviation: 0.03
Immobilized partner concentration (10-6M): 13.5
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 3
Measured pKd: 4.95
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: DGKZ
Fragment: 919-928
Site: PBM
Sequence: IQREDQETAV
Construct: biotin-ttds-IQREDQETAV

Protein B

Protein: SNTG2
Fragment: 70-159
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.27
Immobilized partner concentration (10-6M): 6
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
Measured pKd: 4.88
PUBMED ID: 36115835

Experiment series 3

Protein A

Protein: DGKZ
Fragment: 919-928
Site: PBM
Sequence: IQREDQETAV
Construct: biotin-ttds-IQREDQETAV

Protein B

Protein: SNTG2
Fragment: 70-159
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.41
Normalized holdup BI: 0.46
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.72
PUBMED ID: 36115835
Experimental Details

not reported internal standards of holdup layout #6