Interaction Details


Details of experiments between Q13574 and Q96L92
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: DGKZ
Fragment: 919-928
Site: PBM
Sequence: IQREDQETAV
Construct: biotin-ttds-IQREDQETAV

Protein B

Protein: SNX27
Fragment: 1-541
Construct: endogenous protein (Jurkat)
Average holdup BI: 0.97
Immobilized partner concentration (10-6M): 10
P value: 6.1
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic binding equation
Number of measurements: 3
Measured pKd: 6.55
PUBMED ID: 37942667
Experimental Details

-log10(P Two-sided unpaired T-test (3 vs 6))

Experiment series 2

Protein A

Protein: DGKZ
Fragment: 919-928
Site: PBM
Sequence: IQREDQETAV
Construct: biotin-ttds-IQREDQETAV

Protein B

Protein: SNX27
Fragment: 1-541
Construct: endogenous protein (Jurkat)
Experiment method: nHU-WB_Jurkat
Details of affinity fitting: hyperbolic binding equation (8 point titration)
Date of measurement: 2022-08-03
Number of measurements: 3
Measured pKd: 6.4
PUBMED ID: 36542723

Experiment series 3

Protein A

Protein: DGKZ
Fragment: 919-928
Site: PBM
Sequence: IQREDQETAV
Construct: biotin-ttds-IQREDQETAV

Protein B

Protein: SNX27
Fragment: 1-541
Construct: endogenous protein (Jurkat)
Experiment method: nHU-WB_Jurkat
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-08-03
Number of measurements: 3
Measured pKd: 6.03
PUBMED ID: 36542723

Experiment series 4

Protein A

Protein: DGKZ
Fragment: 919-928
Site: PBM
Sequence: IQREDQETAV
Construct: biotin-ttds-IQREDQETAV

Protein B

Protein: SNX27
Fragment: 40-141
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.91
Holdup BI standard deviation: 0.03
Normalized holdup BI: 0.91
Normalized holdup BI standard deviation: 0.03
Immobilized partner concentration (10-6M): 13.5
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 4
Measured pKd: 6
PUBMED ID: 36115835

Experiment series 5

Protein A

Protein: DGKZ
Fragment: 919-928
Site: PBM
Sequence: IQREDQETAV
Construct: biotin-ttds-IQREDQETAV

Protein B

Protein: SNX27
Fragment: 40-141
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.72
Immobilized partner concentration (10-6M): 6
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
Measured pKd: 5.91
PUBMED ID: 36115835

Experiment series 6

Protein A

Protein: DGKZ
Fragment: 1-928
Construct: endogenous protein (Jurkat)

Protein B

Protein: SNX27
Fragment: 40-141
Site: PDZ
Construct: avi(biotin)-his6-MBP-TEVsite-PDZ
Average holdup BI: 0.87
Immobilized partner concentration (10-6M): 10
P value: 3.05
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-08-03
Number of measurements: 3
Measured pKd: 5.82
PUBMED ID: 36542723
Experimental Details

-log10(P Two-sided unpaired T-test (6 vs 3))

Experiment series 7

Protein A

Protein: DGKZ
Fragment: 919-928
Site: PBM
Sequence: IQREDQETAV
Construct: biotin-ttds-IQREDQETAV

Protein B

Protein: SNX27
Fragment: 40-141
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.9
Normalized holdup BI: 0.9
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 5.81
PUBMED ID: 36115835
Experimental Details

not reported internal standards of holdup layout #6

Experiment series 8

Protein A

Protein: DGKZ
Fragment: 1-928
Construct: endogenous protein (Jurkat)

Protein B

Protein: SNX27
Fragment: 40-141
Site: PDZ
Construct: avi(biotin)-his6-MBP-TEVsite-PDZ
Average holdup BI: 0.78
Immobilized partner concentration (10-6M): 10
P value: 4.74
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-06-27
Number of measurements: 6
Measured pKd: 5.55
PUBMED ID: 36542723
Experimental Details

-log10(P Two-sided unpaired T-test (6 vs 6))