Interaction Details


Details of experiments between Q13224 and Q96RT1
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: GRIN2B
Fragment: 1475-1484
Site: PBM
Sequence: EKLSSIESDV
Construct: Biotin-Ahx-EKLSSIESDV

Protein B

Protein: LAP2
Fragment: 1314-1412
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.22
Holdup BI standard deviation: 0
Normalized holdup BI: 0.24
Normalized holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 2
Measured pKd: 4.26
PUBMED ID: 36115835