Interaction Details


Details of experiments between Q13153 and Q9NYP3
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: PAK1
Fragment: 1-545
Construct: endogenous proteins (Jurkat)

Protein B

Protein: DONSON
Fragment: 71-88
Construct: biotin-ttds-GPAAARRNPFARLDNRPR-CONH2
Average holdup BI: -0.06
Immobilized partner concentration (10-6M): 10
P value: 0.34
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic equation
Date of measurement: 2024-04-11
Number of measurements: 6
The affinity was below the detection threshold of the assay.
Experimental Details

Batch = Helsinki_batch7; Ncontrols = 24; Nsamples = 6; Npreys = 6872; threshold curvature: 4.55

Experiment series 2

Protein A

Protein: PAK1
Fragment: 1-545
Construct: endogenous proteins (Jurkat)

Protein B

Protein: DONSON
Fragment: 71-88
Construct: biotin-ttds-GPAAARRNPFARLDNRPR-CONH2
Average holdup BI: 0.06
Immobilized partner concentration (10-6M): 10
P value: 0.48
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic equation
Date of measurement: 2023-03-15
Number of measurements: 6
The affinity was below the detection threshold of the assay.
Experimental Details

Batch = Helsinki_batch2; Ncontrols = 18; Nsamples = 6; Npreys = 6313; threshold curvature: 1.21